07-582-I

Anti-phospho-IRF-3 (Ser396) Antibody

from rabbit, purified by affinity chromatography

• 동의어(들): Interferon regulatory factor 3, IRF-3
• UNSPSC 코드: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

속성

• 생물학적 소스: rabbit
• Quality Level: 100
• 항체 형태: affinity isolated antibody
• 항체 생산 유형: primary antibodies
• 클론: polyclonal
• 정제법: affinity chromatography
• 종 반응성: human
• 종 반응성(상동성에 의해 예측): porcine (based on 100% sequence homology), rhesus macaque (based on 100% sequence homology)
• 기술: western blot: suitable
• NCBI 수납 번호: NP_001562
• UniProt 수납 번호: Q14653
• 배송 상태: wet ice
• 타겟 번역 후 변형: phosphorylation (pSer396)
• 유전자 정보: human ... IRF3(3661) ; pig ... Irf3(396656) ; rhesus monkey ... Irf3(718970)

설명

일반 설명

Interferon regulatory factors (IRFs) comprise a family of transcription factors that function with the Jak/Stat pathway to regulate interferon (IFN) and IFN-inducible gene expression in response to viral infection. IRF-3 can inhibit cell growth and plays a critical role in controlling the expression of genes in the innate immune response. In unstimulated cells, IRF-3 is present in the cytoplasm. Viral infection results in phosphorylation of IRF-3 and leads to its translocation to the nucleus where it activates promoters containing IRF-3-binding sites. Phosphorylation of IRF-3 occurs at a cluster of C-terminal serine and threonine residues (between 385 and 405) leading to its association with the p300/CBP coactivator protein that promotes DNA binding and transcriptional activity.

특이성

This antibody recognizes IRF-3 phosphorylated at Ser396.

면역원

Epitope: SNS → ANA: Mutation of this residue results in loss of viral-induced phosphorylation of IRF-3.

KLH-conjugated linear peptide corresponding to human IRF-3 phosphorylated at Ser396.

애플리케이션

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Transcription Factors

Use Anti-phospho-IRF-3 (Ser396) Antibody (Rabbit Polyclonal Antibody) validated in WB to detect phospho-IRF-3 (Ser396) also known as Interferon regulatory factor 3.

품질

Evaluated by Western Blot in untreated and sendai virus treated A549 cell lysates.

Western Blot Analysis: A 1:1,000 dilution of this antibody detected phospho-IRF-3 (Ser396) in 10 µg of sendai virus treated A549 cell lysate.

표적 설명

~50 kDa observed. Uncharacterized bands may appear at ~40 kDa and ~80 kDa in some lysates.

결합

Replaces: 07-582

물리적 형태

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

저장 및 안정성

Stable for 1 year at 2-8°C from date of receipt.

분석 메모

Control
Untreated and sendai virus treated A549 cell lysates

기타 정보

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable