추천 제품
생물학적 소스
mouse
Quality Level
항체 형태
affinity isolated antibody
항체 생산 유형
primary antibodies
클론
F6, monoclonal
종 반응성
rat, human, mouse, rabbit
포장
antibody small pack of 25 μg
제조업체/상표
Upstate®
기술
immunocytochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
동형
IgG1
NCBI 수납 번호
배송 상태
ambient
타겟 번역 후 변형
unmodified
유전자 정보
human ... BMI1(648)
일반 설명
The Bmi1 protein is a member of the polycomb group (PcG) proteins. PcG proteins form multiprotein complexes that function as transcriptional repressors.
Several lines of evidence implicate Bmi1 in tumorigenesis. Firstly, Bmi1 cooperates with c-Myc in the generation of lymphomas in double transgenic mice. Secondly, Bmi1 blocks senescence and immortalizes mouse embryo fibroblasts, although not human fibroblasts, and in combination with an activated H-ras gene leads to neoplastic transformation. Thirdly, the BMI1 gene is amplified in certain mantle cell lymphomas and is overexpressed in a subset of non-small cell lung cancer, colorectal carcinomas, multiple myelomas and medulloblastomas, but not glioblastomas. Fourthly, the transformation of human hematopoietic progenitor cells by the oncogenic fusion protein E2a-Pbx1 requires Bmi1. Indeed, based on a list of genes differentially expressed in a mouse model of metastatic prostate cancer on a wild-type and bmi1-deficient genetic background a gene signature was recently identified that may predict response to therapy and survival in multiple types of cancer.
Several lines of evidence implicate Bmi1 in tumorigenesis. Firstly, Bmi1 cooperates with c-Myc in the generation of lymphomas in double transgenic mice. Secondly, Bmi1 blocks senescence and immortalizes mouse embryo fibroblasts, although not human fibroblasts, and in combination with an activated H-ras gene leads to neoplastic transformation. Thirdly, the BMI1 gene is amplified in certain mantle cell lymphomas and is overexpressed in a subset of non-small cell lung cancer, colorectal carcinomas, multiple myelomas and medulloblastomas, but not glioblastomas. Fourthly, the transformation of human hematopoietic progenitor cells by the oncogenic fusion protein E2a-Pbx1 requires Bmi1. Indeed, based on a list of genes differentially expressed in a mouse model of metastatic prostate cancer on a wild-type and bmi1-deficient genetic background a gene signature was recently identified that may predict response to therapy and survival in multiple types of cancer.
특이성
Recognizes Bmi-1 (triplet).
면역원
Immunized with recombinant Bmi-1 protein corresponding to residues 1-202 of mouse Bmi-1.
애플리케이션
Anti-Bmi-1 Antibody, clone F6 is a Mouse Monoclonal Antibody for detection of Bmi-1 also known as Polycomb group RING finger protein 4, RING finger protein 51 and has been validated in ICC, IP & WB.
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
Chromatin Biology
Chromatin Biology
품질
routinely evaluated by immunoblot on RIPA lysates from U2OS cells.
표적 설명
40-44 kDa
물리적 형태
Format: Purified
Immunoaffinity Purified immunoglobulin in Protein A Purified immunoglobulin in 0.1M Tris-Glycine, 0.15M NaCl, 0.05% sodium azide, pH 7.4.
Protein G Purified
저장 및 안정성
Stable for 1 year at 2-8°C from date of receipt.
분석 메모
Control
U2OS whole cell lysate, K-562 nuclear extract, 0-4 hour embryos or 12-24 hour embryos
U2OS whole cell lysate, K-562 nuclear extract, 0-4 hour embryos or 12-24 hour embryos
기타 정보
Please refer to lot specific datasheet.
법적 정보
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
면책조항
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
John K Mich et al.
eLife, 3, e02669-e02669 (2014-05-21)
Neurosphere formation is commonly used as a surrogate for neural stem cell (NSC) function but the relationship between neurosphere-initiating cells (NICs) and NSCs remains unclear. We prospectively identified, and isolated by flow cytometry, adult mouse lateral ventricle subventricular zone (SVZ)
Ruimin Huang et al.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 25(12), 4138-4149 (2011-08-23)
The BMI1 gene is overexpressed in ≈ 90% of human neuroblastomas. However, little is known about the regulation of BMI1 expression. Using microarray and immunohistochemical analysis, we show that BMI1 expression correlated with MYCN levels in MYCN-amplified human neuroblastomas, and
Large Litter Rearing Enhances Leptin Sensitivity and Protects Selectively Bred Diet-Induced Obese Rats from Becoming Obese.
Patterson CM, Bouret SG, Park S, Irani BG, Dunn-Meynell AA, Levin BE
Endocrinology null
Polycomb-group complex 1 acts as an E3 ubiquitin ligase for Geminin to sustain hematopoietic stem cell activity.
Motoaki Ohtsubo,
Proceedings of the National Academy of Sciences of the USA null
Bmi-1 is a novel molecular marker of nasopharyngeal carcinoma progression and immortalizes primary human nasopharyngeal epithelial cells
Song, Li-Bing, et al
Cancer Research, 66, 6225-6232 (2006)
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