05-1075

Anti-FOXO1 Antibody, clone 2H8.2

clone 2H8.2, from mouse

• 동의어(들): Forkhead box protein O1A, Forkhead in rhabdomyosarcoma, forkhead box O1, forkhead box O1A (rhabdomyosarcoma), forkhead homolog in rhabdomyosarcoma
• UNSPSC 코드: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

속성

• 생물학적 소스: mouse
• Quality Level: 100
• 항체 형태: purified antibody
• 항체 생산 유형: primary antibodies
• 클론: 2H8.2, monoclonal
• 종 반응성: rat, human, mouse
• 포장: antibody small pack of 25 μg
• 기술: immunocytochemistry: suitable; immunohistochemistry: suitable (paraffin); western blot: suitable
• 동형: IgG1κ
• NCBI 수납 번호: NP_002006.2
• UniProt 수납 번호: Q12778
• 배송 상태: ambient
• 타겟 번역 후 변형: unmodified
• 유전자 정보: human ... FOXO1(2308) ; mouse ... Foxo1(56458)

설명

일반 설명

Forkhead box O (FOXO) transcription factors play an important role in modulating metabolic functions. Given the relatively high expression of FOXO1 in insulin-responsive tissues, this transcription factor is highly poised to regulate energy metabolism. When nutrient and insulin levels are low, FOXO1 promotes expression of gluconeogenic enzymes. Conversely, in the fed state, insulin levels rise and stimulate uptake of glucose primarily into skeletal muscle and other organs, including adipose tissue. Under certain pathophysiologic conditions, including insulin resistance, negative signaling to FOXO1 is compromised. (Gross, D.N., 2009).

특이성

This antibody detects FOXO1.
FOXO1 shuttles between the nucleus and the cytosol.

면역원

GST-tagged recombinant protein corresponding to full length FOXO1.

애플리케이션

Immunocytochemistry: A 1:500 dilution of a previous lot was used.

Immunocytochemistry: Representative lot data.
A 1:500 dilution of a previous lot was used.

Immunohistochemistry (Paraffin): Representative lot data.
Optimal Staining With Epitope Retrieval: Ductal Carcinoma In Situ.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Transcription Factors

Use Anti-FOXO1 Antibody, clone 2H8.2 (Mouse Monoclonal Antibody) validated in WB, ICC, IHC(P) to detect FOXO1 also known as Forkhead box protein O1A, Forkhead in rhabdomyosarcoma.

품질

Evaluated by western blot on HEK293 cell lysate.
Western Blot Analysis: 2 µg/mL of this antibody detected FOXO1 in HEK293 cell lysate.

표적 설명

~78-82 kDa

결합

Replaces: 04-1005

물리적 형태

Format: Purified

Protein G Purified

Purified mouse monoclonal in buffer containing 0.1M Tris-Glycine (pH 7.4), 150mM NaCl with 0.05% sodium azide.

저장 및 안정성

Stable for 1 year at 2-8ºC from date of receipt.

분석 메모

Control
HEK293 cell lysate

기타 정보

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.