04-1570-I

Anti-RNA polymerase II subunit B1 (phospho-CTD Ser-7) Antibody, clone 4E12

culture supernatant, clone 4E12, from rat

• 동의어(들): DNA-directed RNA polymerase II subunit RPB1, RNA polymerase II subunit B1, DNA-directed RNA polymerase II subunit A, DNA-directed RNA polymerase III largest subunit, RNA-directed RNA polymerase II subunit RPB1
• UNSPSC 코드: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

속성

• 생물학적 소스: rat
• Quality Level: 100
• 항체 형태: culture supernatant
• 항체 생산 유형: primary antibodies
• 클론: 4E12, monoclonal
• 종 반응성: mouse
• 종 반응성(상동성에 의해 예측): human (based on 100% sequence homology)
• 기술: ChIP: suitable; ELISA: suitable; western blot: suitable
• 동형: IgG1κ
• NCBI 수납 번호: NP_000928
• UniProt 수납 번호: P24928
• 배송 상태: wet ice
• 타겟 번역 후 변형: phosphorylation (pSer7)
• 유전자 정보: human ... POLR2B(5431)

설명

일반 설명

RPB1 (RNA polymerase II subunit B1) is the catalytic component of RNA polymerase II which synthesizes mRNA and non-coding RNAs. During transcription, elongation is influenced by the phosphorylation status of the C-terminal domain (CTD) of RPB1, which acts as an assembly platform for factors regulating transcription initiation, elongation, termination and mRNA processing. Phosphorylation occurs mainly at residues ′Ser-2′ and ′Ser-5′ of the tandem 7 residue repeats in the C-terminal domain (CTD), which activates Pol II. This phosphorylation also can occur at ′Ser-7′ of the heptapepdtide repeat. This antibody recognizes the ′Ser-7′ CTD residue of RPB1.

특이성

Demonstrated to react with human RBP1 in Ni et al., 2011. Transcription 2:5, 237-242

This antibody recognizes RNA polymerase II subunit B1 phosphorylated at Ser7 of the C-terminal domain (CTD).

면역원

Epitope: Phosphorylated Ser7 of the C-terminal domain (CTD)

Ovalbumin-conjugated linear peptide corresponding to human RNA polymerase II subunit B1 phosphorylated at Ser7 of the C-terminal domain (CTD).

애플리케이션

Anti-RNA polymerase II subunit B1 (phospho-CTD Ser-7) Antibody, clone 4E12 is a highly specific rat monoclonal antibody & that targets RNA Polymerase & has been tested in western blotting.

Chromatin Immunoprecipitation Analysis: A representative lot was used by an independent laboratory to immunoprecipitate RNA polymerase II subunit B1 (phospho-CTD Ser-5) in ChIP (Chapman, R., et al. (2007). Science. 318(5857):1780-1782.).
Demonstrated to react with human RBP1 in Ni et al., 2011. Transcription 2:5, 237-242

품질

Evaluated by Western Blotting in untreated and lambda phosphatase treated NIH/3T3 cell lysate.

Western Blotting Analysis: A 1:2,000 dilution from a representative lot detected RNA polymerase II subunit B1 (phospho-CTD Ser-7) in 10 μg of untreated NIH/3T3 cell lysate.

표적 설명

~220 kDa observed

결합

Replaces: 04-1570

물리적 형태

Rat monoclonal IgG1κ with 0.05% sodium azide.

안전성 정보

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 1