05-1410-M
Anti-CD45 Antibody, clone F10-89-4
clone F10-89-4, from mouse, purified by affinity chromatography
동의어(들):
CD45 antigen, Leukocyte common antigen precursor, SCID due to PTPRC deficiency, T200 glycoprotein, T200 leukocyte common antigen, Human homolog of severe combined immunodeficiency due to PTPRC deficiency, Leukocyte-common antigen, Protein tyrosine phosph
About This Item
추천 제품
생물학적 소스
mouse
Quality Level
항체 형태
affinity isolated antibody
항체 생산 유형
primary antibodies
클론
F10-89-4, monoclonal
정제법
affinity chromatography
종 반응성
mouse
종 반응성(상동성에 의해 예측)
human, rat
기술
flow cytometry: suitable
immunofluorescence: suitable
immunohistochemistry: suitable (paraffin)
immunoprecipitation (IP): suitable
western blot: suitable
동형
IgG2a
NCBI 수납 번호
UniProt 수납 번호
타겟 번역 후 변형
unmodified
유전자 정보
human ... PTPRC(5788)
일반 설명
특이성
The LCA comprises of at least 5 isoforms ranging from 180-220 kDa which are produced as a result of alternative splicing of exons A, B or C. CBL 124 recognizes epitopes common to
all of the isoforms.
Antigen distribution:
Thymocytes >95%
Granulocytes >95%
Monocytes >95%
B cells (CD20+) >95%
T cells (CD3+) >95%
NK cells (CD16+) >95%
Peripheral blood lymphocytes >95%
FUSION PARTNER: NS1 myeloma cell line
면역원
애플리케이션
Immunohistochemistry (paraffin): A previous lot of this antibody was used in the discrimination between malignant cells of haematopoietic origin and other malignancies in frozen tissue sections.
Immunoprecipitation: A previous lot of this antibody was used in studies of the leucocyte common antigen complex and its identification by the Western blotting technique.
Flow Cytometry: Studies of CD45 expression and the association with T-cell receptor signaling was performed using this antibody of previous lot.
Studies of the mechanism by which CD45 regulates B cell activation through protein tyrosine phospatases.
Optimal working dilutions must be determined by the end user.
Immunohistochemistry(paraffin): Representative testing from a previous lot.
Optimal Staining of CD45 Monoclonal Antibody: Tonsil
Signaling
Immunological Signaling
품질
Western Blot Analysis: 1:500 dilution of this lot detected CD45 on 10 μg of Jurkat lysates
표적 설명
결합
물리적 형태
We recommend that each laboratory determine an optimum working titre for use in its particular application.
저장 및 안정성
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
분석 메모
Jurkat cell lysate
기타 정보
면책조항
적합한 제품을 찾을 수 없으신가요?
당사의 제품 선택기 도구.을(를) 시도해 보세요.
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 2
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
자사의 과학자팀은 생명 과학, 재료 과학, 화학 합성, 크로마토그래피, 분석 및 기타 많은 영역을 포함한 모든 과학 분야에 경험이 있습니다..
고객지원팀으로 연락바랍니다.