04-1564

Anti-Estrogen Receptor α Antibody, clone F3-A

ascites fluid, clone F3-A, from mouse

• 동의어(들): Estradiol receptor, Nuclear receptor subfamily 3 group A member 1, estrogen receptor 1, estrogen receptor alpha, estrogen receptor alpha delta 4*,5,6,7*/633 isoform, estrogen receptor alpha delta 6,7 isoform
• UNSPSC 코드: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

속성

• 생물학적 소스: mouse
• Quality Level: 100
• 항체 형태: ascites fluid
• 항체 생산 유형: primary antibodies
• 클론: F3-A, monoclonal
• 종 반응성: rat, human, mouse
• 기술: immunocytochemistry: suitable; western blot: suitable
• 동형: IgG1κ
• NCBI 수납 번호: NP_000116
• UniProt 수납 번호: P03372
• 배송 상태: wet ice
• 타겟 번역 후 변형: unmodified
• 유전자 정보: human ... ESR1(2099)

설명

일반 설명

Estrogen Receptor α (ERα) is a 65 kDa nuclear hormone receptor. This steroid hormone receptor is involved in the regulation of eukaryotic gene expression and affects cellular proliferation and differentiation in target tissues. ERα is a ligand-activated transcription factor and upon being bound by a ligand such as estrogen, a conformational change occurs that allows dimerization and binding to an estrogen response element. When bound to DNA, Estrogen Receptor α can positively or negatively regulate gene transcription. Like other steroid hormone receptors, Estrogen Receptors are intracellular proteins. Two ERs are known, ERα, classically known as ER, and ERβ. The Estrogen Receptor is believed to play an important role in the growth and development of a subset of hormone dependent human breast cancers. ERβ is expressed in prostate, ovary, and other reproductive organs. Although its steroid specificity is very similar to that of the ERα, the molecular mechanisms regulating the transcriptional activity of ERβ may be distinct from those regulating ERα.

특이성

This antibody recognizes Estrogen Receptor α.

면역원

Epitope: Unknown

Linear peptide corresponding to human Estrogen Receptor α.

애플리케이션

Anti-Estrogen Receptor α Antibody, clone F3-A is a mouse monoclonal antibody for detection of Estrogen Receptor α also known as Estradiol receptor & Nuclear receptor subfamily 3 group A member 1 has been validated in WB, ICC.

Immunocytochemistry Analysis: 1:500 dilution from a representative lot detected Estrogen Receptor α in A431 cells.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Transcription Factors

품질

Evaluated by Western Blot in mouse ovary tissue lysate.

Western Blot Analysis: 1:1,000 dilution of this antibody detected Estrogen Receptor α on 10 µg of mouse ovary tissue lysate.

표적 설명

~ 67 kDa

물리적 형태

Unpurified

Unpurified mouse monoclonal IgG1κ preservative-free ascites.

저장 및 안정성

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

분석 메모

Control
Mouse ovary tissue lysate

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: nwg
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable