06-935

Anti-Estrogen Receptor α Antibody

from rabbit, purified by affinity chromatography

• 동의어(들): ER-alpha, Estradiol receptor, Nuclear receptor subfamily 3 group A member 1
• UNSPSC 코드: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

속성

• 생물학적 소스: rabbit
• Quality Level: 100
• 항체 형태: affinity isolated antibody
• 항체 생산 유형: primary antibodies
• 클론: polyclonal
• 정제법: affinity chromatography
• 종 반응성: human
• 종 반응성(상동성에 의해 예측): mouse (based on 100% sequence homology), rat (immunogen homology)
• 기술: immunocytochemistry: suitable; immunohistochemistry: suitable (paraffin); immunoprecipitation (IP): suitable; western blot: suitable
• NCBI 수납 번호: NP_036821
• UniProt 수납 번호: P06211
• 배송 상태: wet ice
• 타겟 번역 후 변형: unmodified
• 유전자 정보: human ... ESR1(2099) ; mouse ... Esr1(13982) ; rat ... Esr1(24890)

설명

일반 설명

Estrogen Receptor alpha is a 65 kDa protein and a member of the steroid family of nuclear receptors. Estrogen Receptor alpha is a ligand-activated transcription factor that, when bound to estrogen, induces a conformational change that allows dimerization and binding to estrogen response element sequences. When bound to DNA, Estrogen Receptor alpha can positively or negatively regulate gene transcription. Like other steroid hormone receptors, Estrogen Receptors are intracellular proteins. Estrogen Receptors play an important role in regulating mammary gland growth and differentiation. Breast tumors that are ER positive may also show a more favorable response to anti-estrogen therapies.

특이성

This antibody recognizes the C-terminus of Estrogen Receptor alpha.

면역원

Epitope: C-terminus.

KLH-conjugated linear peptide corresponding to the C-terminus of rat Estrogen Receptor alpha.

애플리케이션

• Tested applications
• Immunohistochemistry (Paraffin) Analysis: A 1:400 dilution from a representative lot detected Estrogen Receptor in human stomach, human uterine cancer, human breast cancer and human cervical cancer tissue sections.
• Immunoprecipitation Analysis: 4 µg from a representative lot immunoprecipitated Estrogen Receptor in MCF-7 cell lysate.
• Immunocytochemistry Analysis: A 1:500 dilution from a representative lot detected Estrogen Receptor in MCF-7 cells.
• Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user. "

Anti-Estrogen Receptor , Cat. No. 06-935, is a rabbit polyclonal antibody that detects Estrogen Receptor and is tested for use in Western Blotting, Immunocytochemistry, Immunoprecipitation, and Immunohistochemistry (Paraffin).

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Hormones & Receptors

품질

Evaluated by Western Blotting in MCF-7 cell lysate. Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Estrogen Receptor in MCF-7 cell lysate.

표적 설명

~58 kDa observed. UniProtKB/Swiss-Prot P06211 (ESR1_RAT) lists a molecular weight of 67 kDa A molecular weight between 52-58 kDA due to variant isoforms may be observed in some cell lysates (Al-Bader, M.D. (2006). Reprod Biol Endocrinol. 4:13.; Geisler, J.G. et al. (2002). Diabetes. 51(7):2158-2169.). Uncharacterized bands may be seen at a molecular weight of ~10 kDa, ~35 kDa, ~41 kDa, ~105 kDa, and ~220 kDa in some cell lysates.

물리적 형태

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

저장 및 안정성

Stable for 1 year at 2-8°C from date of receipt.

분석 메모

Control
MCF7 cell lysate

기타 정보

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable