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  • Role of dissolved oxygen availability on lactobionic acid production from whey by Pseudomonas taetrolens.

Role of dissolved oxygen availability on lactobionic acid production from whey by Pseudomonas taetrolens.

Bioresource technology (2012-02-09)
Saúl Alonso, Manuel Rendueles, Mario Díaz
要旨

The influence of dissolved oxygen availability on cell growth and lactobionic acid production from whey by Pseudomonas taetrolens has been investigated for the first time. Results from pH-shift bioreactor cultivations have shown that high agitation rate schemes stimulated cell growth, increased pH-shift values and the oxygen uptake rate by cells, whereas lactobionic acid production was negatively affected. Conversely, higher aeration rates than 1.5 Lpm neither stimulated cell growth nor lactobionic acid production (22% lower for an aeration rate of 2 Lpm). Overall insights into bioprocess performance enabled the implementation of 350 rpm as the optimal agitation strategy during cultivation, which increased lactobionic productivity 1.2-fold (0.58-0.7 g/Lh) compared to that achieved at 1000 rpm. Oxygen supply has been shown to be a key bioprocess parameter for enhanced overall efficiency of the system, representing essential information for the implementation of lactobionic acid production at a large scale.

材料
製品番号
ブランド
製品内容

Sigma-Aldrich
ラクトビオン酸, 97% (TLC)
カタログ番号
容量
在庫状況
単価
数量
Sigma-Aldrich
ラクトビオン酸, ≥97% (TLC), cell impermeant agent
カタログ番号
容量
在庫状況
単価
数量
ラクトビオン酸, European Pharmacopoeia (EP) Reference Standard
カタログ番号
容量
在庫状況
単価
数量