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  • Human fetal membrane-mesenchymal stromal cells generate functional spinal motor neurons in vitro.

Human fetal membrane-mesenchymal stromal cells generate functional spinal motor neurons in vitro.

iScience (2022-10-15)
Giulia Gaggi, Andrea Di Credico, Simone Guarnieri, Maria Addolorata Mariggiò, Patrizia Ballerini, Angela Di Baldassarre, Barbara Ghinassi
要旨

Human fetal membrane mesenchymal stromal cells (hFM-MSCs) are a cell population easily isolable from the amniochorionic membrane of term placentas, without ethical issues or safety limitations. We previously reported that hFM-MSCs share some epigenetic characteristics with pluripotent stem cells and can overcome the mesenchymal commitment. Here, we demonstrated that hFM-MSCs can give rise to spinal motor neurons by the sequential exposure to specific factors that induced a neuralization, caudalization and ventralization of undifferentiated cells, leading to a gradual gene and protein upregulation of early and late MN markers. Also, spontaneous electrical activity (spikes and bursts) was recorded. Finally, when co-cultured with myotubes, differentiated MNs were able to create functional neuromuscular junctions that induced robust skeletal muscle cell contractions. These data demonstrated the hFM-MSCs can generate a mature and functional MN population that may represent an alternative source for regenerative medicine, disease modeling or drug screening.

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Sigma-Aldrich
Smoothened Agonist, SAG, A cell-permeable Smoothened Agonist, SAG, CAS 364590-63-6, modulates the coupling of Smo with its downstream effector by interacting with the Smo heptahelical domain (KD = 59 nM).
Sigma-Aldrich
抗-βチューブリンIII(神経)抗体、マウスモノクローナル マウス宿主抗体, ~1.0 mg/mL, clone 2G10, purified from hybridoma cell culture