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  • Transcription of a yeast phosphoglucomutase isozyme gene is galactose inducible and glucose repressible.

Transcription of a yeast phosphoglucomutase isozyme gene is galactose inducible and glucose repressible.

Molecular and cellular biology (1990-04-01)
D Oh, J E Hopper
要旨

The Saccharomyces cerevisiae GAL5 (PGM2) gene was isolated and shown to encode the major isozyme of phosphoglucomutase. Northern (RNA) blot hybridization revealed that the GAL5 transcript level increased three- to fourfold in response to galactose and was severely repressed in response to glucose. Total cellular phosphoglucomutase activity was likewise responsive to galactose and to glucose, and this responsiveness was found to be due primarily to variation in the activity of the major isozyme of phosphoglucomutase. These results imply that the major and minor isozymes of phosphoglucomutase have distinct roles in yeast cells. The galactose inducibility of GAL5 was found to be under the control of the GAL4, GAL80, and GAL3 genes. In striking contrast to other galactose-inducible genes, the GAL5 gene exhibited an unusually high GAL4-independent basal level of expression. These results have implications for metabolic trafficking.

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Sigma-Aldrich
グルコース-6-リン酸デヒドロゲナーゼ パン酵母(S. cerevisiae種)由来, Type IX, lyophilized powder, 200-400 units/mg protein (modified Warburg-Christian)