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Merck

Exopolysaccharide Quantification for the Plant Pathogen Ralstonia solanacearum.

Bio-protocol (2017-05-20)
Rémi Peyraud, Timothy P Denny, Stéphane Genin
要旨

Soluble exopolysaccharide is a major virulence factor produced by the plant pathogen Ralstonia solanacearum. Its massive production during plant infection is associated with the arrest of water flow in xylem vessels leading eventually to plant death. The composition of this heavy macromolecule includes mainly N-acetylgalactosamine. Here we describe a colorimetric method for quantitative determination of the soluble exopolysaccharide present in culture supernatant of R. solanacearum.

材料
製品番号
ブランド
製品内容

Sigma-Aldrich
リン酸カリウム 一塩基性, powder, suitable for cell culture, suitable for insect cell culture, suitable for plant cell culture, ≥99.0%
カタログ番号
容量
在庫状況
単価
数量
Sigma-Aldrich
硫酸アンモニウム, for molecular biology, ≥99.0%
カタログ番号
容量
在庫状況
単価
数量
Sigma-Aldrich
L-グルタミン酸 一ナトリウム塩 一水和物, ≥98.0% (NT)
カタログ番号
容量
在庫状況
単価
数量