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  • Characterising hyperinsulinemia-induced insulin resistance in human skeletal muscle cells.

Characterising hyperinsulinemia-induced insulin resistance in human skeletal muscle cells.

Journal of molecular endocrinology (2020-01-29)
Mark C Turner, Neil R W Martin, Darren J Player, Richard A Ferguson, Patrick Wheeler, Charlotte J Green, Elizabeth C Akam, Mark P Lewis
要旨

Hyperinsulinaemia potentially contributes to insulin resistance in metabolic tissues, such as skeletal muscle. The purpose of these experiments was to characterise glucose uptake, insulin signalling and relevant gene expression in primary human skeletal muscle-derived cells (HMDCs), in response to prolonged insulin exposure (PIE) as a model of hyperinsulinaemia-induced insulin resistance. Differentiated HMDCs from healthy human donors were cultured with or without insulin (100 nM) for 3 days followed by an acute insulin stimulation. HMDCs exposed to PIE were characterised by impaired insulin-stimulated glucose uptake, blunted IRS-1 phosphorylation (Tyr612) and Akt (Ser473) phosphorylation in response to an acute insulin stimulation. Glucose transporter 1 (GLUT1), but not GLUT4, mRNA and protein increased following PIE. The mRNA expression of metabolic (PDK4) and inflammatory markers (TNF-α) was reduced by PIE but did not change lipid (SREBP1 and CD36) or mitochondrial (UCP3) markers. These experiments provide further characterisation of the effects of PIE as a model of hyperinsulinaemia-induced insulin resistance in HMDCs.

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DL-グリセルアルデヒド 3-リン酸 溶液, 45-55 mg/mL in H2O
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抗GLUT-1抗体、CT, from rabbit, purified by affinity chromatography
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抗リン酸化IRS1 (Tyr608) マウス/ (Tyr612) ヒト抗体, Upstate®, from rabbit
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Anti-IRS1 Antibody, clone 58-10C-31, rabbit monoclonal, clone 58-10C-31, from rabbit
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