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  • DL-3-n-butylphthalide promotes dendrite development in cortical neurons subjected to oxygen-glucose deprivation/reperfusion.

DL-3-n-butylphthalide promotes dendrite development in cortical neurons subjected to oxygen-glucose deprivation/reperfusion.

Cell biology international (2018-04-27)
Peng Zhang, Ruxiang Xu, Yang Guo, Jiazhen Qin, Yiwu Dai, Ning Liu, Cuiying Wu
要旨

The limited degree of functional recovery is closely associated with the condition of the periinfarct cortex after ischemic stroke. The model of oxygen-glucose deprivation in cultured neurons could partly simulate this condition. Proper dendritic morphology is crucial for the correct wiring of neuronal function. Hence, the question of how to facilitate the plasticity of neural dendrites is of great significance. DL-3-n-butylphthalide is an efficient medication for ischemic stroke. In this study, in addition to having neuroprotective effects, DL-3-n-butylphthalide could increase the number of primary and secondary dendrites and of dendritic tips as confirmed by Sholl analysis. This study further demonstrated that DL-NBP inactivates PI3K/AKT signaling to positively regulate dendritic branching.

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Sigma-Aldrich
ラミニン EHS肉腫基底膜由来, 1-2 mg/mL in Tris-buffered saline, 0.2 μm filtered, BioReagent, suitable for cell culture
Sigma-Aldrich
ウシ血清アルブミン ウシ血清由来, lyophilized powder, ≥96% (agarose gel electrophoresis)
Sigma-Aldrich
パラホルムアルデヒド, powder, 95%
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ポリ-L-リシン 臭化水素酸塩, mol wt 15,000-30,000 by viscosity
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LY-294,002 塩酸塩, solid, ≥98% (HPLC)