Roche

Genomics Research – PCR, qPCR, RT-PCR, RT-qPCR, and DIG-Labeling Reagents and Protocols

An essential technique within virtually every molecular biology lab, polymerase chain reaction (PCR) is an easy and affordable method for amplifying specific fragments of DNA by several orders of magnitude. Our specialized kits have been optimized for a variety of PCR applications. Enabling room temperature PCR reaction setup with hot start PCR reagents, FastStart^tm Taq DNA Polymerase is also compatible with automated setups.

Whether you need Kapa Biosystems PCR products for DNA extraction and amplification, or Kapa qPCR and qRT-PCR products utilizing SYBR® Green reagents, we have your PCR needs covered. The SYBR^® Green-based qPCR reagent uses fluorescent dye that binds to double-stranded DNA molecules in order for researchers to quantify the amount of DNA that has been amplified. Probe-based qPCR reagents, such as FastStart^tm Universal Probe Master (ROX), is suitable for use on all real-time PCR instruments that normalize with ROX. For RT-PCR/qRT-PCR applications, the Transcriptor One-Step RT-PCR Kit is designed for fast, sensitive, and specific end-point RT-PCR analysis of RNA. Additional nucleic acid labeling applications are readily met with the DIG-System for membrane hybridization and in situ hybridization needs.

Proteomics Analysis – Protein Stabilization Reagents and Protocols

To maintain the structure and integrity of a protein, it is essential to prevent degradation or aggregation. This can be achieved through the addition of protease and phosphatase inhibitors to buffers and solutions. Widely cited, cOmplete™ and PhosSTOP™ protease and phosphatase inhibitors from Roche are easy-to-use, flexible tablet-based formulations which inhibit a wide range of proteases and phosphatases respectively. With the cOmplete™ Protease Inhibitor Cocktail, each of the water-soluble tablets contains a broad blend of metalloproteases, serine, and cysteine.

protease inhibitors to ensure the protection of proteins isolated from almost any tissue or cell. Additionally, the PhosSTOP™ Phosphatase Inhibitor Cocktail tablet provides a quick-dissolving, water-soluble formulation that contains a proprietary blend of acid and alkaline phosphatase inhibitors, serine/threonine phosphatase inhibitors and tyrosine protein phosphatase inhibitors.

Cell Research – Cell Function, Cell Isolation, and Transfection Reagents and Protocols

Involving the introduction of DNA or RNA into eukaryotic cells, transfection is a widely used technique for the study and modulation of gene expression. Created to afford high transfection efficiency, ease-of-use and reproducibility, X-tremeGENE™ Transfection Reagents provide increased viable cells and more physiologically relevant results for both transfection and siRNA reagents to knock-down gene expression. While many techniques are used to generate data from heterogeneous cell populations, it is often more informative to separate and analyze individual cellular sub-types. Collagenase and Liberase™ Research Grade enzymes are suitable for use with a broad range of cell and tissue types. Additionally, understanding cellular function also highly informative for evaluating cellular health or suggesting whether a potential drug candidate may be cytotoxic. The Roche biochemical reagents portfolio includes optimized ELISA kits for the measurement of cellular proliferation and cell death.