G006141

HPLC Analysis of MAb on Proteomix^® SCX-NP5 Comparing Different Buffer Systems and pH Gradients

application for HPLC

特性

• テクニック: HPLC: suitable
• 試験パラメータ: column: Proteomix SCX-NP5, 25 cm x 4.6 mm I.D., 5 μm particles (Z777161); mobile phase: Mobile phase A: (A) 20 mM MES, pH 6.0; (B) A + 1 M NaCl, pH 6.0Mobile phase B: (A) 2.4 mM Tris, 1.5 mM imidazole, 11.6 mM piperazine, pH 6.0; (B) A, pH 10.5; gradient: Gradient A: 7% B held for 2 min; to 14% B in 20 min, Gradient B: 0 to 30% B in 2 min; to 55% B in 30 min; flow rate: 0.8 mL/min; detector: UV, 280 nm; injection: 5 μL; sample: 10 mg/mL
• 適合性: application for HPLC
• アプリケーション: life science and biopharma; pharmaceutical (small molecule)

詳細

法的情報

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