テクニック
HPLC: suitable
試験パラメータ
column: Ascentis Express C18, 10 cm x 2.1 mm I.D., 2.7 μm particles (53823-U)
mobile phase: [A] water; [B] methanol; [C] acetonitrile; (720:120:120, A:B:C) containing 0.780 g/L potassium bromide, 230 μL/L nitric acid
flow rate: 0.400 mL/min
pressure: 4568 psi (315 bar)
column temp.: 35 °C
detector: FLD excitation = 360 nm, emission = 440 nm
injection: 20 μL
sample: 200 μL spiked 84:16 acetonitrile:di water in 880 μL di water, Spike concentrations: B1 = 16 ppb; B2 = 4 ppb; G1 = 16 ppb; B2 = 4 ppb
適合性
application for HPLC
アプリケーション
food and beverages
アナリシスノート
Aflatoxins are a complex of closely related toxins produced as secondary metablolites of fungi. They are found in grains and nuts such as corn, wheat, or peanuts. They are produced by aspergillus flavus and a. Parasiticus. Members of the group include aflatoxin b1, aflatoxin b2, aflatoxin g1, aflatoxin g2, aflatoxin m1, and aflatoxin m2.
法的情報
Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany
アナライト
詳細
Aflatoxin G2
Aflatoxin G1
Aflatoxin B2
Aflatoxin B1
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