G005890

HPLC Analysis of Paclitaxel and Related Compounds on Ascentis^® Express F5

application for HPLC

特性

• テクニック: HPLC: suitable
• 試験パラメータ: column: Ascentis Express F5, 15 cm x 4.6 mm I.D., 5 μm particles (50631-U); mobile phase: [A] water; [B] acetonitrile; (60:40, A:B); flow rate: 1.5 mL/min; pressure: 2727 psi (188 bar); column temp.: 30 °C; detector: UV, 227 nm; injection: 5 μL; sample: taxols 25 μg/mL in 70:15:15, water:methanol:acetonitrile
• 適合性: application for HPLC
• アプリケーション: pharmaceutical (small molecule)

詳細

アナリシスノート

An efficient reverse phase HPLC method for the HPLC analysis of of Paclitaxel (Taxol) and related compounds is developed using the Ascentis^® Express F5 5μm Fused Core HPLC column. Paclitaxel is a natural product with antitumor activity. TAXOL (paclitaxel) is obtained via a semi-synthetic process from Taxus baccata.
The developed method can be easily used for the routine quality control of bulk and Parenterals dosage form of Paclitaxel within a short analysis time. This method offers the advantages of higher efficies, reduced backpressure and shorter run times over a traditionally run methods over 5 μm totally porous particle columns.

法的情報

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

アナライト

Baccatin III, ≥95% (HPLC)
Cephalomannine, ≥97% (HPLC), solid
Paclitaxel, from Taxus yannanensis, powder