テクニック
HPLC: suitable
試験パラメータ
column: CHIROBIOTIC V, 25 cm x 4.6 mm I.D., 5 μm particles (11024AST)
mobile phase: [A] ethanol; [B] hexane; (25:75, A:B)
flow rate: 1 mL/min
column temp.: 25 °C
detector: UV, 205 nm
injection: 10 μL
sample: 1 mg/mL in methanol
適合性
application for HPLC
アプリケーション
pharmaceutical (small molecule)
アナリシスノート
Methylphenobarbital (also known as mephobarbital and mephobarbitone) is a barbiturate derivative used primarily as an anticonvulsant, but also as a sedative and anxiolytic. It is a chiral compound and the enantiomers have been shown to exhibit different bioactivity, one narcotic, one convulsant, neither of which is seen in the racemate. Shown here is the rapid, efficient separation of methylphenobarbital enantiomers on an Astec CHIROBIOTIC V column. Highest grade HPLC solvents were used to supply low background interference and low particulate contamination for robust, trouble-free operation. A Sigma-Aldrich USP reference standard provided reliable identification and quantification.
法的情報
Astec is a registered trademark of Merck KGaA, Darmstadt, Germany
CHIROBIOTIC is a registered trademark of Sigma-Aldrich Co. LLC
アナライト
詳細
Mephobarbital, United States Pharmacopeia (USP) Reference Standard
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