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製品名
TSKgel® Boronate-5PW HPLC Column, phase m-aminophenyl boronate, L × I.D. 5 cm × 5 mm, 10 μm particle size, glass
物質
glass
製品種目
TSKgel®
アナライトの化学物質クラス
proteins
メーカー／製品名
Tosoh 14449
パラメーター
≤20% organic solvent
≤3 M salt concentration
>0 °C temperature
1.2 mL/min flow rate
30 bar max. pressure
テクニック
HPLC: suitable
L × 内径
5 cm × 5 mm
Matrix
hydroxylated methacrylic polymer
マトリックス活性基
m-aminophenyl boronate phase
粒径
10 μm
使用pH
2-9
キャパシティ
40 μmol/mL, resin adsorption capacity (sorbitol)
分離法
affinity
保管温度
2-8°C
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詳細
High efficiency, resin-based TSKgel affinity columns (10 μm particles) separate or purify many enzymes and other proteins.
Coupling of m-aminophenyl boronate to TSKgel 5PW polymeric support results in a ligand capable of forming a tetrahedral boronate anion under alkaline pH conditions. This anionic structure can bind with 1,2 cis-diol groups such as those found in carbohydrates, carbohydrate containing compounds and catecholamines. Interaction between the boronate anion and the 1,2 cis-diol groups is enhanced in the presence of Mg2+ ions and is inhibited by amine-containing buffers. Adsorption onto the TSKgel Boronate-5PW matrix takes place in basic buffers such as HEPES and morpholine while desorption takes place in carbohydrate or amine-containing mobile phase like sorbitol or Tris.
Applications include: nucleic acids, catecholamines, nucleosides and nucleotides.
Coupling of m-aminophenyl boronate to TSKgel 5PW polymeric support results in a ligand capable of forming a tetrahedral boronate anion under alkaline pH conditions. This anionic structure can bind with 1,2 cis-diol groups such as those found in carbohydrates, carbohydrate containing compounds and catecholamines. Interaction between the boronate anion and the 1,2 cis-diol groups is enhanced in the presence of Mg2+ ions and is inhibited by amine-containing buffers. Adsorption onto the TSKgel Boronate-5PW matrix takes place in basic buffers such as HEPES and morpholine while desorption takes place in carbohydrate or amine-containing mobile phase like sorbitol or Tris.
Applications include: nucleic acids, catecholamines, nucleosides and nucleotides.
法的情報
TSKgel is a registered trademark of Tosoh Corporation
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