コンテンツへスキップ
Merck
すべての画像(4)

主要文書

安全性情報

ZRB2538

Sigma-Aldrich

Anti-TRIM14 Antibody, clone 1J17 ZooMAb® Rabbit Monoclonal

newenhanced validation

recombinant, expressed in HEK 293 cells

別名:

Tripartite motif-containing protein 14

ログイン組織・契約価格を表示する


About This Item

UNSPSCコード:
12352203

由来生物

rabbit

品質水準

リコンビナント

expressed in HEK 293 cells

結合体

unconjugated

抗体製品の状態

purified antibody

抗体製品タイプ

primary antibodies

クローン

1J17, recombinant monoclonal

詳細

recombinant, expressed in HEK 293 cells

製品種目

ZooMAb® learn more

フォーム

lyophilized

分子量

calculated mol wt 49.77 kDa
observed mol wt ~50 kDa

精製方法

using Protein A

化学種の反応性

human

包装

antibody small pack of 25

強化検証

recombinant expression
Learn more about Antibody Enhanced Validation

テクニック

affinity binding assay: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable
western blot: suitable

アイソタイプ

IgG

エピトープ配列

N-terminal half

タンパク質IDアクセッション番号

UniProtアクセッション番号

輸送温度

ambient

保管温度

2-8°C

遺伝子情報

human ... TRIM14(9830)

詳細

ZooMAb® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them.

特異性

Clone 1J17 is a ZooMAb® recombinant rabbit monoclonal antibody that specifically detects TRIM14. It targets an epitope within 21 amino acids from the N-terminal half.

免疫原

KLH-conjugated linear peptide corresponding to 21 amino acids from the N-terminal half of human TRIM14.

アプリケーション

Quality Control Testing



Evaluated by Western Blotting in HeLa cell lysate.



Western Blotting Analysis (WB): A 1:1,000 dilution of this antibody detected TRIM14 in HeLa cell lysate.

Tested Applications

Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected TRIM14 in human liver tissue sections.

Affinity Binding Assay: A representative lot of this antibody bound TRIM14 peptide with at least one thousand-fold (1,000X) higher than with non-specific control peptide.

Immunocytochemistry Analysis: A 1:1,000 dilution from a representative lot detected TRIM14 in HeLa cells.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

ターゲットの説明

Tripartite motif-containing protein 14 (UniProt: Q14142) is encoded by the TRIM14 (also known as KIAA0129) gene (Gene ID: 9830) in human. The protein encoded by TRIM14 is a member of the tripartite motif (TRIM) family. It plays an essential role in the innate immune defense against viruses and bacteria. TRIM14 includes zinc-binding domain (B-box type) and two coiled-coil regions. The B-box zinc finger is responsible for inhibition of SPI1-mediated transcriptional activation. It lacks the N-terminal RING domain, which is found in most other proteins in the TRIM family. TRIM14 expressed in various tissues with highest expression in liver and also in immune-related tissues such as the spleen, thymus, and peripheral blood leukocytes and is undetectable in skeletal muscle. The protein localizes to cytoplasmic bodies; mitochondria and cytoplasmic vesicle (phagosomes). Four alternatively spliced transcript variants for this gene have been described. It is involved in various cellular processes and key functions include Antiviral defense, Autophagy, Inflammatory Response and Cell Proliferation and Survival. TRIM14 is crucial in the host defense against viral infections. Its antiviral activity is significant in the context of infections by viruses such as HIV, influenza, and hepatitis B and C. The antiviral mechanism is by IFN-stimulated genes and Cytokine Signaling in Immune system. TRIM14 is also known to be associated with Spondyloepimetaphyseal Dysplasia of Geneviève Type. TRIM14 may contribute to the pathogenesis of autoimmune diseases if its regulatory mechanisms are disrupted. This ZooMAb® recombinant monoclonal antibody, generated by our proprietary technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals.

物理的形状

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.

再構成

300 µg/mL after reconstitution at 25 µL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

保管および安定性

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

法的情報

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

適切な製品が見つかりませんか。  

製品選択ツール.をお試しください

保管分類コード

11 - Combustible Solids

WGK

WGK 2

引火点(°F)

Not applicable

引火点(℃)

Not applicable


適用法令

試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。

Jan Code

ZRB2538-25UL:
ZRB2538-4X25UL:


最新バージョンのいずれかを選択してください:

試験成績書(COA)

Lot/Batch Number

申し訳ございませんが、現在この製品のCOAをオンラインで入手できません。

サポートが必要な場合は、お問い合わせください カスタマーサポート

以前この製品を購入いただいたことがある場合

文書ライブラリで、最近購入した製品の文書を検索できます。

文書ライブラリにアクセスする

ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.

製品に関するお問い合わせはこちら(テクニカルサービス)