ZMS1051

Anti-Dystrophin Antibody, clone 2C6 (MANDYS106) ZooMAb^® Mouse Monoclonal

recombinant, expressed in HEK 293 cells

• 別名: Anti-BMD, Anti-DXS142, Anti-DXS164, Anti-DXS206, Anti-DXS230, Anti-DXS239, Anti-DXS268, Anti-DXS269, Anti-DXS270, Anti-DXS272, Anti-MRX85
• UNSPSCコード: 12352203
• NACRES: NA.41

特性

• 由来生物: mouse
• 品質水準: 200
• リコンビナント: expressed in HEK 293 cells
• 結合体: unconjugated
• 抗体製品の状態: purified antibody
• 抗体製品タイプ: primary antibodies
• クローン: C26, recombinant monoclonal; monoclonal
• 製品種目: ZooMAb^® learn more
• 形状: lyophilized
• 分子量: calculated mol wt ~426.75 kDa; observed mol wt ~425 kDa
• 精製方法: using protein G
• 化学種の反応性: human
• 化学種の反応性（ホモロジーによる予測）: monkey
• 包装: antibody small pack of 25 μL
• 環境により配慮した代替製品の特徴: Waste Prevention; Designing Safer Chemicals; Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• 強化検証: recombinant expression; Learn more about Antibody Enhanced Validation
• sustainability: Greener Alternative Product
• テクニック: immunofluorescence: suitable; immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable; western blot: suitable
• アイソタイプ: IgG2aκ
• UniProtアクセッション番号: P11532
• 環境により配慮した代替製品カテゴリ: Aligned,
• 輸送温度: ambient
• 保管温度: 2-8°C
• ターゲットの翻訳後修飾: unmodified

詳細

詳細

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.

Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.

特異性

Clone C26 is a ZooMAb^® mouse recombinant monoclonal antibody that specifically detects Dystrophin. It targets an epitope from the internal region.

免疫原

TrpE-tagged recombinant fragment corresponding to 110 amino acids from the internal region of human Dystrophin (from the Exon 43-coded pectrin-like repeat 16 region).

アプリケーション

Quality Control Testing

Evaluated by Western Blotting in Human Skeletal Muscle tissue lysate.

Western Blotting Analysis: A 1:10,000 dilution of this antibody detected Dystrophin in Human Skeletal Muscle tissue lysate.

Western Blotting Analysis: A 1:10,000 dilution from a representative lot detected Dystrophin in Human heart tissue lysate.

Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected Dystrophin in human skeletal muscle tissue sections.

Immunofluorescence Analysis: A 1:100 dilution from a representative lot detected Dystrophin in human skeletal muscle tissue sections.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Anti-Dystrophin, clone 2C6 (MANDYS106) ZooMAb^®, Cat. No. ZMS1051, is a recombinant Mouse monoclonal antibody that specifically targets Dystrophin and is tested for use in Immunofluorescence, Immunohistochemistry (Paraffin), and Western Blotting.

ターゲットの説明

Dystrophin (UniProt: P11532; also known as DMD) is encoded by the DMD gene (Gene ID: 1756) in human. Dystrophin is a rod-shaped cytoplasmic protein and a vital part of a protein complex that connects the cytoskeleton of a muscle fiber to the surrounding extracellular matrix through the cell membrane. It is expressed in muscle fibers accumulating in the costameres of myoplasm at the sarcolemma. Its expression is also reported in brain, muscle, kidney, lung and testis. Dystrophin is localized to the inner part of the muscle fiber cell membrane (sarcolemma), where it forms the dystrophin-associated glycoprotein complex (DGC) that links the extracellular matrix to the actin cytoskeleton. The N-terminus is the actin-binding domain, and the carboxy-terminal domain interacts with beta-dystroglycan as well as dystrobrevin and the syntrophin. The central rod domain that comprises the major mass of the dystrophin molecule forms a flexible, rod-shaped structure. Dystrophin plays an important role in stabilizing the muscle fiber against the mechanical forces of muscle contraction by providing a shock-absorbing connection between the cytoskeleton and the extracellular matrix. The DMD gene is the largest known gene in humans and mutations in this gene are known to cause Duchenne muscular dystrophy, a sex-linked recessive disorder that affects males aged 3 to 7 year as proximal muscle weakness with steady progression. This ZooMAb^® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals.

物理的形状

Purified recombinant mouse monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

再構成

300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

保管および安定性

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

法的情報

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 11 - Combustible Solids
• WGK: WGK 1
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable