ZMS1021

Anti-Influenza A Nucleoprotein Antibody, clone A3 ZooMAb^® Mouse Monoclonal

recombinant, expressed in HEK 293 cells

• UNSPSCコード: 12352203
• NACRES: NA.41

特性

• 由来生物: mouse
• 品質水準: 200
• リコンビナント: expressed in HEK 293 cells
• 結合体: unconjugated
• 抗体製品の状態: purified antibody
• 抗体製品タイプ: primary antibodies
• クローン: A3, recombinant monoclonal; monoclonal
• 製品種目: ZooMAb^® learn more
• フォーム: lyophilized
• 分子量: calculated mol wt ~56.21 kDa; observed mol wt ~56 kDa
• 化学種の反応性: Influenza A virus subtype H1N1
• 包装: antibody small pack of 25 μL
• 環境により配慮した代替製品の特徴: Waste Prevention; Designing Safer Chemicals; Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• 強化検証: recombinant expression; Learn more about Antibody Enhanced Validation
• sustainability: Greener Alternative Product
• テクニック: ELISA: suitable; immunocytochemistry: suitable; western blot: suitable
• アイソタイプ: IgG1
• UniProtアクセッション番号: O76090
• 環境により配慮した代替製品カテゴリ: , Aligned
• 輸送温度: ambient
• 保管温度: 2-8°C
• ターゲットの翻訳後修飾: unmodified

詳細

詳細

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.

Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.

特異性

Clone A3 is a ZooMAb^® mouse recombinant monoclonal antibody that specifically detects Influenza A virus nucleoprotein.

免疫原

Influenza A virus H1N1.

アプリケーション

Quality Control Testing

Evaluated by Western Blotting with recombinant nucleoprotein from Influenza A virus H1N1.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected recombinant nucleoprotein from Influenza A virus H1N1.

Tested Applications

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Influenza A Nucleoprotein in MDCK cells infected with Influenza A virus.

ELISA Analysis: 1.0 μg/mL from a representative lot detected recombinant nucleoprotein from Influenza A virus.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Anti-Influenza A Nucleoprotein, clone A3 ZooMAb^®, Cat. No. ZMS1021, is a recombinant Mouse monoclonal antibody that detects Influenza A virus nucleoprotein and is tested for use in ELISA and Immunocytochemistry, and Western Blotting.

ターゲットの説明

Bestrophin-1 (UniProt: O76090; also known as TU15B, Vitelliform macular dystrophy protein 2) is encoded by the BEST1 (also known as VMD2) gene (Gene ID: 7439) in human. Bestrophin-1 is a multi-pass, homopentameric membrane protein that contains three cytoplasmic domains, three extracellular domains, and four transmembrane domains. It is predominantly expressed in the basolateral membrane of the retinal pigment epithelium. However, its expression has also been reported in cortical and hippocampal astrocytes and in cerebellar Bergmann glia and lamellar astrocytes. It serves as a calcium-activated anion channel and its calcium-binding clasp consists of the acidic cluster of amino acids Glu300, Asp301, Asp302, Asp303, and Asp4304). Under physiological conditions, Bestrophin-1 is also involved in glutamate and GABA release from astrocytes to modulate neuronal excitability, synaptic transmission, and synaptic plasticity. Mutations in BEST1 gene have been linked to various macular dystrophies and retinitis pigmentosa that is characterized by retinal pigment deposits and primary loss of photoreceptor cells followed by secondary loss of cone photoreceptors. This ZooMAb^® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Oh, S-J., et al. (2017). Exp. Neurobiol. 26(3); 113-121).

物理的形状

Purified recombinant mouse monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

再構成

300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

保管および安定性

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

法的情報

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 11 - Combustible Solids
• WGK: WGK 1
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable