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由来生物
mouse
リコンビナント
expressed in E. coli
アッセイ
≥98% (HPLC)
≥98% (SDS-PAGE)
形状
lyophilized
分子量
21.9 kDa
包装
pkg of 20 μg
テクニック
cell culture | mammalian: suitable
不純物
<0.1 EU/μg endotoxin, tested
色
white to off-white
タンパク質IDアクセッション番号
UniProtアクセッション番号
輸送温度
wet ice
保管温度
−20°C
遺伝子情報
mouse ... TNFSF13(69583)
詳細
Research area: Immunology and Cytokines
APRIL (a proliferation-inducing ligand) was identified in 1998 through database mining and is a member of the TNF (tumor necrosis factor) family. APRIL gene is localized to human chromosome 17p13, contains six exons, and is alternatively transcribed to 1.8, 2.1, and 2.4 kb mRNA transcripts. APRIL protein is composed of a cytosolic domain of 28 amino acids, and a transmembrane region. It also contains an exoplasmic region, made up of a stalk and a TNF domain. It shares the highest similarity to BLyS (B lymphocyte stimulator) protein, ~30% sequence similarity in the TNF domain. It is synthesized as a type II transmembrane protein and is proteolytically processed as a mature protein, which is a soluble and non-covalent trimer. Recombinant murine APRIL is a soluble 21.9 kDa protein, consisting of 192 amino acid residues.
APRIL (a proliferation-inducing ligand) was identified in 1998 through database mining and is a member of the TNF (tumor necrosis factor) family. APRIL gene is localized to human chromosome 17p13, contains six exons, and is alternatively transcribed to 1.8, 2.1, and 2.4 kb mRNA transcripts. APRIL protein is composed of a cytosolic domain of 28 amino acids, and a transmembrane region. It also contains an exoplasmic region, made up of a stalk and a TNF domain. It shares the highest similarity to BLyS (B lymphocyte stimulator) protein, ~30% sequence similarity in the TNF domain. It is synthesized as a type II transmembrane protein and is proteolytically processed as a mature protein, which is a soluble and non-covalent trimer. Recombinant murine APRIL is a soluble 21.9 kDa protein, consisting of 192 amino acid residues.
生物化学的／生理学的作用
A proliferation-inducing ligand (APRIL) and its ligand interaction activate several signaling pathways, including nuclear factor-kappa B (NF-κB), p38, c-Jun N-terminal kinase mitogen-activated protein kinase (JNK MAPK), and Protein Kinase B/Forkhead Box O (AKT/FOXO), which are necessary for promoting the survival and differentiation of B cells and plasma cells. Additionally, APRIL signaling aids in the regulation of cytokine production, the generation of humoral immunity, decreases T cell-dependent antibody responses, and facilitates IgA class switching. These functions highlight the crucial role of APRIL in regulating immune responses. Furthermore, APRIL is also known to induce immune cell apoptosis leading to immunosuppression, and tumor development.
シーケンス
MRREVSRLQR SGGPSQKQGE RPWQSLWEQS PDVLEAWKDG AKSRRRRAVL TQKHKKKHSV LHLVPVNITS KDSDVTEVMW QPVLRRGRGL EAQGDIVRVW DTGIYLLYSQ VLFHDVTFTM GQVVSREGQG RRETLFRCIR SMPSDPDRAY NSCYSAGVFH LHQGDIITVK IPRANAKLSL SPHGTFLGFV KL
物理的形状
凍結乾燥品(10 mM酢酸ナトリウム、pH 5.0 + 100 mMアルギニン)。
再構成
バイアルは、開封前に遠心してください。0.1～1.0 mg/mLの濃度となるよう水で再構成してください。ボルテックスは行わないでください。この溶液は2～8°Cで1週間まで保存できます。長期保存の際は、キャリアタンパク質(0.1% BSAなど)を含有するバッファーで希釈し、1回使用量ごとに分けて-20～-80°Cで保存することをお勧めします。
保管分類コード
11 - Combustible Solids
WGK
WGK 3
引火点(°F)
Not applicable
引火点(℃)
Not applicable
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
Jan Code
SRP3189-20UG:
試験成績書（COA）
製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
An APRIL to remember: novel TNF ligands as therapeutic targets.
Dillon SR, et al.
Nature Reviews. Drug Discovery, 5(3), 235-246 (2006)
Tarlan Mamedov et al.
PloS one, 14(3), e0213438-e0213438 (2019-03-13)
A plant expression platform with eukaryotic post-translational modification (PTM) machinery has many advantages compared to other protein expression systems. This promising technology is useful for the production of a variety of recombinant proteins including, therapeutic proteins, vaccine antigens, native additives
Tumor necrosis factor superfamily member APRIL contributes to fibrotic scar formation after spinal cord injury.
Funk LH. et al.
Journal of Neuroinflammation, 13(1) (2016)
Fabienne Mackay et al.
Cytokine & growth factor reviews, 14(3-4), 311-324 (2003-06-06)
B cell activating factor belonging to the TNF family (BAFF) and apoptosis-inducing ligand (APRIL) are two related members of the TNF ligand superfamily. Although they share two receptors, TACI and BCMA, transgenic and knockout mice in this system reveal that
G Yu et al.
Nature immunology, 1(3), 252-256 (2001-03-23)
We report that the tumor neurosis factor homolog APRIL (a proliferation-inducing ligand) stimulates in vitro proliferation of primary B and T cells and increases spleen weight due to accumulation of B cells in vivo. APRIL functions via binding to BCMA
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