SRP0734

PIP4K2B, Active

PRECISIO^® Kinase, recombinant

• UNSPSCコード: 12352202
• NACRES: NA.27

特性

• リコンビナント: expressed in baculovirus infected Sf9 cells
• アッセイ: 70-95% (SDS-PAGE)
• 形状: frozen liquid
• 比活性: 4500-6000 nmol/min·mg (ADP-Glo)
• 分子量: ~73 kDa
• 濃度: 0.1 μg/μL
• アクセッション番号: NM_003559
• 輸送温度: dry ice
• 保管温度: −70°C
• 遺伝子情報: human ... PIP4K2B(8396)

詳細

アプリケーション

Useful for kinase assay.

生物化学的／生理学的作用

Recombinant full-length human PIP4K2B was expressed by Baculovirus in Sf9 insect cells using an N-terminal GST tag. PIP4K2B (phosphatidylinositol-5-phosphate 4-kinase, type II, beta) catalyzes the phosphorylation of phospha-tidylinositol-5-phosphate on the fourth hydroxyl of the myo-inositol ring to form phosphatidylinositol-5,4-bisphosphate. PIP4K2B is a member of the phosphatidylinositol-5-phosphate 4-kinase family. PIP5K2B interacts specifically with the juxtamembrane region of the p55 TNF receptor and that PIP5K2B activity is increased in mammalian cells by treatment with TNF-alpha ⁽¹⁾. PIP5P4KB plays a role in determining insulin sensitivity and adiposity ⁽²⁾.

再構成

Formulated in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, with 25% glycerol.

保管および安定性

Store in aliquots at -70°C. Avoid freeze/thaw cycles.

法的情報

PRECISIO is a registered trademark of Merck KGaA, Darmstadt, Germany

安全性情報

• 保管分類コード: 10 - Combustible liquids
• WGK: WGK 1
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable