由来生物
human
リコンビナント
expressed in baculovirus infected Sf9 cells
アッセイ
≥40% (SDS-PAGE)
フォーム
aqueous solution
分子量
60 kDa
包装
pkg of 10 μg
保管条件
avoid repeated freeze/thaw cycles
濃度
0.4 mg/mL
NCBIアクセッション番号
輸送温度
dry ice
保管温度
−70°C
遺伝子情報
human ... CERK(64781)
詳細
Ceramide kinase (CERK) is a 537-amino acid protein. It has a catalytic region, a putative amino-myristoylation site in its amino terminal region and a pleckstrin homology domain. The gene encoding CERK is localized on human chromosome 22q13.31.
Human ceramide kinase (CERK) (GenBank Accession No. NM_022766), 1-537 (end) with C-terminal Flag-tag, MW= 60 kDa, expressed in Baculovirus expression system.
アプリケーション
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
生物化学的／生理学的作用
Ceramide kinase (CERK) is an enzyme involved in sphingolipid metabolism and in the synthesis of ceramide-1-phosphate. It associates with calmodulin and at the time of Fc receptor-mediated phagocytosis, it aggregates in lipid rafts. CERK may have a role in calcium signaling. The protein functions in the phagocytosis of polymorphonuclear leukocytes and degranulation of mast cells.
単位の定義
One unit is defined as the amount of enzyme required to produce 1 pmole of ceramide-1-phosphate/min at 37°C.
保管分類コード
10 - Combustible liquids
WGK
WGK 3
引火点(°F)
Not applicable
引火点(℃)
Not applicable
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
カルタヘナ法
カルタヘナ法
Jan Code
SRP0339-10UG:
最新バージョンのいずれかを選択してください：
A conserved cysteine motif essential for ceramide kinase function
Lidome E, et al.
Biochimie, 90(10), 1560-1565 (2008)
Ceramide kinase: a novel lipid kinase molecular cloning and functional characterization
Sugiura M, et al.
The Journal of Biological Chemistry (2002)
Identification of genes associated with tumorigenesis of retinoblastoma by microarray analysis
Chakraborty S, et al.
Genomics, 90(3), 344-353 (2007)
Tack-Joong Kim et al.
Biochemical and biophysical research communications, 342(2), 611-617 (2006-02-21)
Ceramide kinase (CERK) converts ceramide to ceramide-1-phosphate (C1P), which has recently emerged as a new bioactive molecule capable of regulating diverse cellular functions. The N-terminus of the CERK protein encompasses a sequence motif known as a pleckstrin homology (PH) domain.
Tomomi Date et al.
In vitro cellular & developmental biology. Animal, 43(10), 321-323 (2007-10-25)
Ceramide kinase (CerK) has important roles in leukocyte functions, including the role in degranulation of mast cells and the phagocytosis of polymorphonuclear leukocytes, so its expression levels should be strictly regulated. Here, we report that the mRNA expression and enzyme
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