リコンビナント
expressed in baculovirus
アッセイ
≥70% (SDS-PAGE)
フォーム
aqueous solution
比活性
≥20 units/μg
分子量
47.1 kDa
NCBIアクセッション番号
輸送温度
dry ice
保管温度
−70°C
遺伝子情報
mouse ... SPHK1(8877)
詳細
Mouse Sphingosine kinase 1 (GenBank Accession No. AF415213), full length with N-terminal His tag, MW = 47.1 kDa, expressed in a Baculovirus infected Sf9 cell expression system.
アプリケーション
Sphingosine kinase 1a is useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. Sphingosine kinase 1a has been used in a study to assess the design of sphingosine kinase inhibitors to promote apoptosis of chemotherapeutic resistant cancer cells. Sphingosine kinase 1a has also been used in a study to investigate novel biomarkers for early prostate cancer detection.
単位の定義
One unit of sphingosine kinase activity is defined as the amount of enzyme required to produce 1 pmol of S1P/min
物理的形状
Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 50% glycerol and 3 mM DTT.
調製ノート
氷上で解凍してください。最初に解凍した際、チューブを軽く遠心して内容物を完全に回収してください。酵素液は一回の使用量ずつ分注してください。分注された希釈前の酵素を-70°Cで保存してください。注:酵素は凍結解凍の繰り返しに非常に弱いので、注意してください。
最新バージョンのいずれかを選択してください:
Keng Gat Lim et al.
The international journal of biochemistry & cell biology, 44(9), 1457-1464 (2012-05-29)
Sphingosine kinase 1 catalyses the formation of the bioactive lipid, sphingosine 1-phosphate and is a target for anti-cancer agents. We demonstrate here that 2-(p-hydroxyanilino)-4-(p-chlorophenyl)thiazole (SKi, also referred to as SKI-II), FTY720 (Fingolimod), and (S)-FTY720 vinylphosphonate inhibit sphingosine kinase 1 activity
J Nunes et al.
British journal of cancer, 106(5), 909-915 (2012-02-09)
Current markers available for screening normal populations and for monitoring prostate cancer (PCa) treatment lack sensitivity and selectivity. Sphingosine-1-phosphate (S1P) is a circulating lipid second messenger involved in cell growth and migration, the immune response, angiogenesis, and malignant transformation. Eighty-eight
Brian M Barth et al.
Journal of neuroscience research, 90(1), 229-242 (2011-09-21)
Inflammation accompanied by severe oxidative stress plays a vital role in the orchestration and progression of neurodegeneration prevalent in chronic and acute central nervous system pathologies as well as in aging. The proinflammatory cytokine tumor necrosis factor-α (TNFα) elicits the
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