SRE0117

Salt Active Nuclease (SAN)

High Purity, recombinant, expressed in Pichia pastoris, Triton-free

• 別名: endonuclease, salt active endonuclease, salt tolerant nuclease
• UNSPSCコード: 12352204
• NACRES: NA.54

特性

• リコンビナント: expressed in Pichia pastoris
• 品質水準: 200
• 組成: Triton-free
• 包装: pkg of 25 kU
• 輸送温度: dry ice
• 保管温度: −20°C

詳細

詳細

This Salt Active Nuclease (SAN) is the ultimate solution for efficient removal of nucleic acids in manufacturing and bioprocessing workflows. This nonspecific, recombinant endonuclease has optimum activity at high salt concentrations, which can improve efficiency and yield in various workflows.

アプリケーション

• Useful for purification of tagged recombinant proteins
• Manufacturing of nucleic acid-free molecular enzymes and reagents
• Research of vaccines, viruses and antibodies

特徴および利点

• Active at low temperatures
• Binds strongly to cation exchange media because of high isoelectric point, even at elevated pH and salt concentrations where few other proteins show binding
• Typically does not bind to anion exchange or IMAC media
• Detergent free No protease detected

単位の定義

One unit is defined as the amount of enzyme that causes a ΔA260 = 1.0 in 30 minutes at 37 °C in 25 mM Tris-HCI pH 8.5 (@25 °C), 5 mM MgCl₂, 500 mM NaCl, and 50 µg/mL calf thymus DNA.

再構成

In solution

その他情報

• Temperature: 5 – 40°C, optimal: ~35°C
• Salt concentration (NaCl / KCl): 150 - 900 mM, optimal: 400 - 650 mM
• Mg²⁺: >1 mM is required for activity, optimal 5 - 50 mM
• pH: 7.3 – 10.0, optimal 8.2 - 9.2

安全性情報

• 保管分類コード: 12 - Non Combustible Liquids
• WGK: WGK 2
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable