由来生物
rabbit
品質水準
物質
colorless
クローン
8Q8T5, monoclonal
形状
liquid
分子量
28 kDa
化学種の反応性
human
濃度
2.50 mg/mL
テクニック
immunofluorescence: 1:50 - 1:200
immunohistochemistry: 1:50 - 1:200
western blot: 1:500 - 1:2000
色
colorless
アイソタイプ
IgG
免疫原配列
MSIDQRKKMLKNLRNTNYDVFEKICWGLGIEYTFPPLYYRRAHRRFVTKKALCIRVFQETQKLKKRRRALKAAAAAQKQAKRRNPDSPAKAIPKTLKDSQ
UniProtアクセッション番号
輸送温度
wet ice
保管温度
−20°C
ターゲットの翻訳後修飾
unmodified
遺伝子情報
human ... MRPS15(64960)
詳細
Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein that belongs to the ribosomal protein S15P family. The encoded protein is more than two times the size of its E. coli counterpart, with the 12S rRNA binding sites conserved. Between human and mouse, the encoded protein is the least conserved among small subunit ribosomal proteins. Pseudogenes corresponding to this gene are found on chromosomes 15q and 19q.
免疫原
A synthetic peptide corresponding to a sequence within amino acids 158-257 of human MRPS15 (P82914).
PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
アプリケーション
WB, IHC, IF/ICC
保管分類コード
10 - Combustible liquids
WGK
WGK 1
試験成績書(COA)
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