おすすめの製品
由来生物
mouse
品質水準
抗体製品の状態
purified from hybridoma cell culture
抗体製品タイプ
primary antibodies
クローン
FL-D6, monoclonal
形状
buffered aqueous solution
濃度
~1.0 mg/mL
テクニック
immunoblotting: 1.25-2.5 μg/mL using ExtrAvidin-FITC
immunohistochemistry: suitable
indirect ELISA: 0.15-0.3 μg/mL using 5 μg/ml ExtrAvidin-FITC
アイソタイプ
IgG1
輸送温度
dry ice
保管温度
−20°C
ターゲットの翻訳後修飾
unmodified
詳細
Anti-FITC antibody, Mouse monoclonal, (mouse IgG1 isotype) is derived from the FL-D6 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from a BALB/c mouse immunized with FITC (fluorescein isothiocyanate)-BSA conjugate. This antibody specifically recognizes both free and conjugated FITC.
FITC (fluorescein isothiocyanate) is a fluorochrome dye that absorbs ultraviolet or blue light (with peak wavelength of approximately 495 nm) causing molecules to become excited and emit a visible yellow-green light (with peak wavelength of approximately 519 nm).
FITC (fluorescein isothiocyanate) is a fluorochrome dye that absorbs ultraviolet or blue light (with peak wavelength of approximately 495 nm) causing molecules to become excited and emit a visible yellow-green light (with peak wavelength of approximately 519 nm).
特異性
Monoclonal Anti-FITC antibody specifically recognizes both free and conjugated FITC. The antibody does not react with bound or free TRITC (tetramethylrhodamine isothiocyanate).
免疫原
FITC-BSA結合体。
アプリケーション
Anti-FITC antibody, Mouse monoclonal may be used in:
- immunoblotting
- enzyme-linked immunosorbent assay (ELISA)
- immunohistochemistry
生物化学的／生理学的作用
FITC (fluorescein isothiocyanate) is a commonly used protein marker including antibodies in immunofluorescent techniques. Antibodies to FITC are used to identify FITC labeled proteins and as models to study the mechanism of antibody response to a well-defined hapten.
物理的形状
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
保管および安定性
Store at –20°C. For continuous use, store at 2–8°C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation. Discard working dilution samples if not used within 12 hours.
免責事項
Unless otherwise stated in our catalog, our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
適切な製品が見つかりませんか。
製品選択ツール.をお試しください
保管分類コード
10 - Combustible liquids
引火点(°F)
Not applicable
引火点(℃)
Not applicable
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
Jan Code
SAB4200738-BULK:
SAB4200738-100UL:
SAB4200738-VAR:
試験成績書（COA）
製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
J J Haaijman et al.
Histochemistry, 84(4-6), 363-370 (1986-01-01)
Fluorescein (Fl) and tetramethyl rhodamine (Rh) were evaluated as possible candidates for a double hapten sandwich system in enzyme immunohistology. Monoclonal antibodies were raised against Fl and Rh. Their fine-specificity was tested with a competition-like assay. A pair of Mab's
Steven J Metallo et al.
Journal of the American Chemical Society, 125(15), 4534-4540 (2003-04-10)
This paper describes the synthesis of bifunctional polyacrylamides containing pendant vancomycin (Van) and fluorescein groups, and the use of these polymers to direct antibodies against fluorescein to self-assembled monolayers (SAMs) presenting d-alanine-d-alanine (dAdA) groups. These polymers bind biospecifically to these
D Samuel et al.
Journal of immunological methods, 107(2), 217-224 (1988-03-16)
Monoclonal antibodies to FITC were produced and shown to be specific for the fluorochrome. Molecular weight marker proteins labelled with FITC could be detected after SDS-PAGE and transfer onto nitrocellulose using anti-FITC followed by an anti-mouse IgG-alkaline phosphatase conjugate. The
S J Rattle et al.
Clinical chemistry, 30(9), 1457-1461 (1984-09-01)
We describe a novel separation procedure for immunoradiometric assays involving monoclonal antibodies in which both radiolabeled and capture antibodies are used in solution, the capture antibody being labeled with fluorescein isothiocyanate (FITC). Separation is achieved by incubation with anti-FITC antibodies
Elsa Charignon et al.
International journal of molecular sciences, 21(21) (2020-10-28)
We describe here the evaluation of the cytotoxic efficacy of two platinum (II) complexes bearing an N-heterocyclic carbene (NHC) ligand, a pyridine ligand and bromide or iodide ligands on a panel of human metastatic cutaneous melanoma cell lines representing different
ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.
製品に関するお問い合わせはこちら（テクニカルサービス）