SAB4200581

Anti-Msi-1 (N-terminal) antibody produced in rabbit

~1.0 mg/mL, affinity isolated antibody

• 別名: Anti-MSI1, Anti-MSI1H, Anti-MUSASHI 1, Anti-m-Msi-1
• UNSPSCコード: 12352203

特性

• 由来生物: rabbit
• 結合体: unconjugated
• 抗体製品の状態: affinity isolated antibody
• 抗体製品タイプ: primary antibodies
• クローン: polyclonal
• フォーム: buffered aqueous solution
• 分子量: ~39 kDa
• 化学種の反応性: rat, human
• 濃度: ~1.0 mg/mL
• テクニック: immunoblotting: 1-2 μg/mL using extracts of SH-SY5Y cells.; immunohistochemistry: 10-20 μg/mL using formalin-fixed, paraffin-embedded rat cerebellum.; indirect immunofluorescence: 5-10 μg/mL using HeLa cells.
• UniProtアクセッション番号: O43347
• 輸送温度: dry ice
• 保管温度: −20°C
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... MSI1(4440) ; mouse ... Msi1(17690) ; rat ... Msi1(259272)

詳細

詳細

Musashi-1 (MSI1) gene is mapped to human chromosome 12q24. Msi-1 is highly expressed in neural stem/progenitor cells (NS/PCs).

免疫原

Synthetic peptide corresponding to an N-terminal sequence of human Msi-1, conjugated to KLH. The corresponding sequence is identical in rat and mouse Msi-1.

アプリケーション

Anti-Msi-1 (N-terminal) antibody produced in rabbit has been used in:

• immunoblotting
• immunofluorescence
• immunohistochemistry

生物化学的／生理学的作用

Musashi-1 (Msi-1) is a neural RNA-binding protein that regulate translation of target mRNAs. Msi-1 binds to the 3′-untranslated region (UTR) of its target mRNAs in neural stem/progenitor cells (NS/PCs), repressing their translation, and interfering with NS/PC differentiation thus maintaining NP/PCs in the undifferentiated state. It is associated with maintenance and asymmetric cell division of neural stem cells. Loss of Msi-1 function disrupts the balance between germ-line stem cell renewal and differentiation, causing premature differentiation. Msi-1 has been shown to regulate the Notch and Wnt signaling pathway. Upregulation of Msi-1 augments Notch signaling, via translational repression of the Notch signaling antagonist m-Numb, thereby inhibiting the differentiation of neural stem cells into neurons. Expression of Msi1- has been detected in several human cancers including brain tumors, suggesting its role in oncogenic development.

物理的形状

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 10 - Combustible liquids
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable