SAB4200461

Anti-Arc (N-terminal) antibody produced in rabbit

~1.0 mg/mL, affinity isolated antibody

• 別名: 抗活性調節細胞骨格関連タンパク質抗体
• UNSPSCコード: 12352203
• NACRES: NA.41
• 結合体: unconjugated
• application: IHC; IP; WB
• クローン: polyclonal
• 化学種の反応性: rat, human
• citations: 2
• テクニック: immunohistochemistry: 20-30 μg/mL using formalin-fixed, paraffin-embedded rat brain.; immunoprecipitation (IP): 2-4 μg using lysates of HEK-293T cells over-expressing human Arc.; western blot: 1.5-3.0 μg/mL using extracts of HEK-293T cells over-expressing human Arc.

特性

• 由来生物: rabbit
• 結合体: unconjugated
• 抗体製品の状態: affinity isolated antibody
• 抗体製品タイプ: primary antibodies
• クローン: polyclonal
• フォーム: buffered aqueous solution
• 分子量: antigen ~55 kDa
• 化学種の反応性: rat, human
• 強化検証: recombinant expression; Learn more about Antibody Enhanced Validation
• 濃度: ~1.0 mg/mL
• テクニック: immunohistochemistry: 20-30 μg/mL using formalin-fixed, paraffin-embedded rat brain.; immunoprecipitation (IP): 2-4 μg using lysates of HEK-293T cells over-expressing human Arc.; western blot: 1.5-3.0 μg/mL using extracts of HEK-293T cells over-expressing human Arc.
• UniProtアクセッション番号: Q7LC44
• 輸送温度: dry ice
• 保管温度: −20°C
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... ARC(23237) ; rat ... Arc(54323)

詳細

詳細

Activity-regulated cytoskeleton-associated protein (Arc) is a growth factor and immediate-early gene (IEG) that is enriched in the brain. Arc is located in the distal dendrites and spine. The Arc gene is located on the human chromosome at 8q24.3.

特異性

Anti-Arc(N-terminal) specifically recognizes humanand rat Arc.

免疫原

synthetic peptide corresponding to a sequence at the N-terminal region of human Arc, conjugated to KLH. The corresponding sequence is highly conserved (90% sequence identity) in rat and mouse Arc.

アプリケーション

Anti-Arc (N-terminal) antibody produced in rabbit may be used in:

• immunoblotting
• immunoprecipitation
• immunohistochemistry

生物化学的／生理学的作用

Activity-regulated cytoskeleton-associated protein (Arc) induction is required for late long-term potentiation (LTP) and memory consolidation. It also plays a role in LTP-induced cofilin phosphorylation and F-actin stabilization. This protein affects synaptic plasticity. Arc regulates the endocytosis of α-amino-3-hydroxy-5-methyl-4-isoxazole propionic acid (AMPA) receptors through its interaction with endocytic proteins endophilin2/3 and dynamin. It is associated with notch signaling and spine morphology.

物理的形状

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

保管および安定性

For continuous use, store at 2-8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers,is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution bycentrifugation before use. Working dilutions should be discarded if not used within 12 hours.

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 10 - Combustible liquids
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable