SAB4200400

Anti-WIPI-2 (C-terminal) antibody produced in rabbit

~1.0 mg/mL, affinity isolated antibody

• 別名: Anti-ATG18B, Anti-Atg21, Anti-CGI-50, Anti-WD repeat domain, phosphoinositide interacting 2, Anti-WIPI2
• UNSPSCコード: 12352203
• NACRES: NA.41

特性

• 由来生物: rabbit
• 結合体: unconjugated
• 抗体製品の状態: affinity isolated antibody
• 抗体製品タイプ: primary antibodies
• クローン: polyclonal
• 形状: buffered aqueous solution
• 分子量: antigen ~49 kDa
• 化学種の反応性: mouse, rat, human
• 濃度: ~1.0 mg/mL
• テクニック: immunoprecipitation (IP): 2.5-5.0 μg using lysates of rat NRK cells.; western blot: 2-4 μg/mL using whole extracts of human G-361 and NIH-3T3 cells.
• UniProtアクセッション番号: Q9Y4P8
• 輸送温度: dry ice
• 保管温度: −20°C
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... WIPI2(26100) ; mouse ... Wipi2(74781) ; rat ... Wipi2(288498)

詳細

詳細

WIPI-2 belongs to the WIPI subfamily of WD40 repeat proteins. It is widely expressed in a variety of cell lines. The protein is characterized with a 7-bladed propeller structure and contain a conserved motif for interaction with phospholipids. It is a membrane constituent of autophagosomes and the plasma membrane.

免疫原

synthetic peptide corresponding to the C-terminal region of human WIPI-2, conjugated to KLH. The corresponding sequence is identical in mouse and rat.

アプリケーション

Anti-WIPI-2 (C-terminal) antibody produced in rabbit may be used in various immunochemical techniques including

• immunoblotting
• immunoprecipitation
• immunostaining.

生物化学的／生理学的作用

WIPI subfamily proteins acts as a key component of many essential biological functions such as signal transduction, transcription regulation and apoptosis. WIPI-2 is a phosphatidylinositol-3-phosphate binding protein that is needed for starvation induced autophagy. In addition, it is also essential for LC3 lipidation and it is linked to early autophagosomal structures together with Atg16L and ULK1.

物理的形状

Solution in 0.01 M phosphate buffered saline pH 7.4, containing 15 mM sodium azide.

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 10 - Combustible liquids
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable