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由来生物
rabbit
品質水準
結合体
unconjugated
抗体製品の状態
affinity isolated antibody
抗体製品タイプ
primary antibodies
クローン
polyclonal
形状
buffered aqueous solution
分子量
antigen ~150 kDa
化学種の反応性
human
濃度
~1.0 mg/mL
テクニック
immunoprecipitation (IP): 5-10 μg using ysates of HEK-293 cells over expressing human EDC4
indirect immunofluorescence: 2-5 μg/mL using paraformaldehyde fixed NIH-3T3 cells over expressing human EDC4
western blot: 0.5-1 μg/mL using lysates of HEK-293 cells over expressing human EDC4
UniProtアクセッション番号
輸送温度
dry ice
保管温度
−20°C
ターゲットの翻訳後修飾
unmodified
遺伝子情報
human ... EDC4(23644)
mouse ... Edc4(234699)
rat ... Edc4(361399)
詳細
Enhancer of mRNA-decapping protein 4 (EDC4), also known as human enhancer of decapping large subunit (HEDLS) or Ge-1, is characterized with an N-terminal WD40 repeat and a C-terminal domain which interacts with mRNA-decapping enzyme subunit 2 (Dcp2), and mediates EDC4 oligomerization and P-body localization .
The EDC4 gene maps on human chromosome 16q22.1.
特異性
Anti-EDC4 (N-terminal) specifically recognizes human EDC4.
アプリケーション
Anti-EDC4 (N-terminal) antibody has been used in:
- immunoprecipitation
- immunofluorescence
- immunoblotting.
生物化学的／生理学的作用
Human enhancer of mRNA-decapping protein 4 (EDC4) associates with hDcp2 and stimulates hDcp2 activity in vitro as well as mediating the interaction between mRNA-decapping enzyme subunit 1 & 2 (DCP1 and DCP2).
Interaction of processing-body components, EDC4 and Dcp1a is essential for the post-transcriptional regulation of interleukin (IL)-6.
物理的形状
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
保管および安定性
Store at –20 °C. For continuous use, the product may be stored at 2–8 °C for up to one month. For extended storage, freeze in working aliquots at –20 °C. Repeated freezing and thawing is not recommended. Storage in “frost-free” freezers is also not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.
免責事項
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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保管分類コード
10 - Combustible liquids
引火点(°F)
Not applicable
引火点(℃)
Not applicable
試験成績書（COA）
製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
A human microprotein that interacts with the mRNA decapping complex
D'Lima N G, et al.
Nature Chemical Biology, 13(2), 174-174 (2017)
Target-specific requirements for enhancers of decapping in miRNA-mediated gene silencing
Eulalio A, et al.
Genes & Development, 21(20), 2558-2570 (2007)
Jucimara Colombo et al.
Oncology reports, 21(3), 649-663 (2009-02-13)
Laryngeal squamous cell carcinoma is very common in head and neck cancer, with high mortality rates and poor prognosis. In this study, we compared expression profiles of clinical samples from 13 larynx tumors and 10 non-neoplastic larynx tissues using a
Nadia G D'Lima et al.
Nature chemical biology, 13(2), 174-180 (2016-12-06)
Proteomic detection of non-annotated microproteins indicates the translation of hundreds of small open reading frames (smORFs) in human cells, but whether these microproteins are functional or not is unknown. Here, we report the discovery and characterization of a 7-kDa human
Martin Fenger-Grøn et al.
Molecular cell, 20(6), 905-915 (2005-12-21)
Decapping is a key step in mRNA turnover. However, the composition and regulation of the human decapping complex is poorly understood. Here, we identify three proteins that exist in complex with the decapping enzyme subunits hDcp2 and hDcp1: hEdc3, Rck/p54
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