由来生物
mouse
結合体
unconjugated
抗体製品の状態
purified from hybridoma cell culture
抗体製品タイプ
primary antibodies
クローン
3F2/4, monoclonal
フォーム
buffered aqueous solution
分子量
antigen ~250 kDa
交差性
human, mouse
濃度
~1.0 mg/mL
テクニック
immunoprecipitation (IP): suitable
western blot: 0.5-1.0 μg/mL using HeLa cell extracts
アイソタイプ
IgG2a
UniProtアクセッション番号
輸送温度
dry ice
保管温度
−20°C
ターゲットの翻訳後修飾
unmodified
遺伝子情報
human ... CHD4(1108)
詳細
Monoclonal Anti-CHD4 (mouse IgG2a isotype) is derived from the hybridoma 3F2/4 produced by the fusion of mouse myeloma cells and splenocytes from CD-1 mice immunized with a synthetic peptide derived from human chromodomain helicase DNA binding protein 4 (CHD4) conjugated to keyhole limpet hemocyanine (KLH). CHD4 is one of the subunits of Mi-2, an ATP-dependent helicase. It belongs to the sucrose non-fermentable (SNF2)/Rad54 helicase family of proteins. The CHD4 gene is mapped to human chromosome 12p13.31 and is highly conserved among animals and plants.
免疫原
synthetic peptide derived from human CHD4, conjugated to KLH
アプリケーション
Monoclonal Anti-CHD4 antibody produced in mouse has been used in:
- immunoblotting
- enzyme linked immunosorbent assay (ELISA)
- immunoprecipitation
生物化学的／生理学的作用
Chromodomain helicase DNA binding protein 4 (CHD4) interacts with metastasis-associated protein (MTA1/2), histone deacetylase 1 (HDAC1) and RbAp46 in the nucleosome remodelling and deacetylation (NuRD) complex. Interestingly, antibodies against CHD4 have been found to be involved in the onset of dermatomyositis (DM), an autoimmune disease which is a form of idiopathic inflammatory myopathy (IIM). Variation in the gene due to mutation is observed in endometrial cancer. CHD4 regulates homologous recombination and thus maintains genome stability.
物理的形状
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
免責事項
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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保管分類コード
10 - Combustible liquids
引火点(°F)
Not applicable
引火点(℃)
Not applicable
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
Jan Code
SAB4200107-200UL:
SAB4200107-BULK:
SAB4200107-25UL:
SAB4200107-VAR:
最新バージョンのいずれかを選択してください：
UV radiation regulates Mi-2 through protein translation and stability
Burd CJ
Test, 283(50), 34976-34982 (2008)
The human Mi-2/NuRD complex and gene regulation
Denslow SA and Wade PA
Oncogene, 26(37), 5433-5433 (2007)
Detection of recurrent cytogenetic aberrations in multiple myeloma: a comparison between MLPA and iFISH
Zang M, et al.
Testing, 6(33), 34276-34276 (2015)
CHD4 mutations promote endometrial cancer stemness by activating TGF-beta signaling
Li Y, et al.
American Journal of Cancer Research, 8(5), 903-903 (2018)
Jinhua Quan et al.
PloS one, 9(9), e108066-e108066 (2014-09-24)
Loss of the chromatin remodeling ATPase CHD5 has been linked to the progression of neuroblastoma tumors, yet the underlying mechanisms behind the tumor suppressor role of CHD5 are unknown. In this study, we purified the human CHD5 complex and found
ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.
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