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Merck

SAB4200048

Sigma-Aldrich

Anti-E1B-AP5 antibody, Rat monoclonal

clone EAP4A11, purified from hybridoma cell culture

別名:

Anti-Adenovirus E1B 55-KD protein-associated protein 5, Anti-Heterogeneous nuclear ribonucleoprotein U-like 1, Anti-hnRNPUL1, Anti-hnRPUL1, Monoclonal Anti-E1B-AP5 antibody produced in rat

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About This Item

UNSPSCコード:
12352203
NACRES:
NA.41

由来生物

rat

品質水準

結合体

unconjugated

抗体製品の状態

purified from hybridoma cell culture

抗体製品タイプ

primary antibodies

クローン

EAP4A11, monoclonal

形状

buffered aqueous solution

分子量

antigen ~110 kDa

化学種の反応性

bovine, canine, human, rat, monkey, mouse

テクニック

immunocytochemistry: suitable
immunoprecipitation (IP): suitable
western blot: 0.05-0.1 μg/mL using HT-29 cell extracts

アイソタイプ

IgG2b

UniProtアクセッション番号

輸送温度

dry ice

保管温度

−20°C

ターゲットの翻訳後修飾

unmodified

遺伝子情報

関連するカテゴリー

詳細

Monoclonal Anti-E1B-AP5 (rat IgG2b isotype) is derived from the hybridoma EAP4A11 produced by the fusion of mouse myeloma cells (P3X63Ag8.653) and splenocytes from rat immunized with full length human E1B-AP5 fusion protein. E1B-55K-associated protein 5 (E1B-AP5), also known as heterogeneous nuclear ribonucleoprotein U-like 1(hnRNPUL1), is a nuclear RNA-binding protein of the heterogeneous nuclear ribonucleoprotein (hnRNP) family.
The E1B-AP5 gene is located on the human chromosome at 19q13.2.

特異性

Monoclonal Anti-E1B-AP5 recognizes human, monkey, bovine, dog, mouse and rat E1B-AP5.

免疫原

full length human E1B-AP5 fusion protein.

アプリケーション

Monoclonal Anti-E1B-AP5 antibody produced in rat may be used in:
  • immunoblotting
  • immunoprecipitation
  • immunocytochemistry

生物化学的/生理学的作用

E1B-55K-associated protein 5 (E1B-AP5) plays a vital role in ATR signaling. In addition, it may play an important role in nucleocytoplasmic RNA transport, and its function is modulated by E1B55kDa in adenovirus-infected cells. Interestingly, this protein was found to be associated with bromodomain-containing protein, BRD7 in a complex that links chromatin events with mRNA processing at the level of transcriptional regulation. It also activates transcription of glucocorticoid-responsive promoter in the absence of ligand-stimulation.

物理的形状

Solution in 0.01M phosphate buffered saline pH 7.4, containing 15 mM sodium azide.

保管および安定性

Store at –20 °C. For continuous use, the product may be stored at 2-8 °C for up to one month. For extended storage, freeze in working aliquots at –20 °C. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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保管分類コード

10 - Combustible liquids

引火点(°F)

Not applicable

引火点(℃)

Not applicable


適用法令

試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。

Jan Code

SAB4200048-VAR:
SAB4200048-BULK:
SAB4200048-25UL:
SAB4200048-200UL:


試験成績書(COA)

製品のロット番号・バッチ番号を入力して、試験成績書(COA) を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。

以前この製品を購入いただいたことがある場合

文書ライブラリで、最近購入した製品の文書を検索できます。

文書ライブラリにアクセスする

Regulation of transcription by the heterogeneous nuclear ribonucleoprotein E1B-AP5 is mediated by complex formation with the novel bromodomain-containing protein BRD7.
Kzhyshkowska J, et al.
The Biochemical Journal, 371, 385-385 (2003)
Regulation of DNA-End Resection by hnRNPU-like Proteins Promotes DNA Double-Strand Break Signaling and Repair
Polo SE, et al.
Molecular Cell, 45, 505-516 (2012)
The interaction of the hnRNP family member E1B-AP5 with p53
Barral PM, et al.
Febs Letters, 579, 2752-2758 (2005)
S Gabler et al.
Journal of virology, 72(10), 7960-7971 (1998-09-12)
The adenovirus type 5 (Ad5) early 1B 55-kDa protein (E1B-55kDa) is a multifunctional phosphoprotein that regulates viral DNA replication and nucleocytoplasmic RNA transport in lytically infected cells. In addition, E1B-55kDa provides functions required for complete oncogenic transformation of rodent cells
J Kzhyshkowska et al.
The Biochemical journal, 358(Pt 2), 305-314 (2001-08-22)
The heterogeneous nuclear ribonucleoprotein (hnRNP) family includes predominantly nuclear proteins acting at different stages of mRNA metabolism. A characteristic feature of hnRNPs is to undergo post-translational asymmetric arginine methylation catalysed by different type 1 protein arginine methyltransferases (PRMTs). A novel

ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.

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