SAB3701031

Anti-Mouse IgG (Fc specific), F(ab′)₂ fragment, highly cross adsorbed-Biotin antibody produced in goat

affinity isolated antibody, lyophilized powder

• UNSPSCコード: 12352203
• NACRES: NA.46

特性

• 由来生物: goat
• 結合体: biotin conjugate
• 抗体製品の状態: affinity isolated antibody
• 抗体製品タイプ: secondary antibodies
• クローン: polyclonal
• 形状: lyophilized powder
• 化学種の反応性: mouse
• テクニック: immunohistochemistry: suitable; indirect ELISA: suitable; western blot: suitable
• 輸送温度: wet ice
• 保管温度: 2-8°C
• ターゲットの翻訳後修飾: unmodified

詳細

詳細

Immunoglobulin G (IgG) belongs to the immunoglobulin family and is a widely expressed serum antibody. It consists of a γ heavy chain in the constant (C) region. The monomeric 150kDa structure of IgG constitutes two identical heavy chains and two identical light chains with molecular weight of 50kDa and 25kDa, respectively. The primary structure of this antibody also contains disulfide bonds involved in linking the two heavy chains, linking the heavy and light chains and resides inside the chains. IgG is further subdivided into four classes namely, IgG1, IgG2, IgG3, and IgG4 with different heavy chains, named γ1, γ2, γ3, and γ4, respectively. Limited digestion using papain cleaves the antibody into three fragments, two of which are identical and contain the antigen-binding activity. The third fragment does not possess antigen-binding activity and is known as fragment crystallizable (Fc). It interacts with cells and effector molecules. The Fc fragment contains the CH2 and CH3 domains of the antibody molecule. Pepsin cleaves the carboxy-terminal side of the disulfide bonds in the general region of the antibody and this gives rise to the F(ab′)2 fragment. The two antigen-binding arms of the antibody are linked in this fragment. Maternal IgG is the only antibody transported across the placenta to the fetus. It passively immunizes the infants.

特異性

This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation. Assay by immunoelectrophoresis resulted in a single precipitin arc against Anti-Biotin, Anti-Goat Serum, Mouse IgG, Mouse IgG F(c) and Mouse Serum. No reaction was observed against Anti-Pepsin, Anti-Goat IgG F(c), Mouse IgG F(ab′)2 or Bovine, Horse and Human Serum Proteins.

免疫原

Mouse IgG F(c) fragment

物性

抗体フォーマット：IgG F(ab')2

物理的形状

0.02 M リン酸カリウム、0.15 M 塩化ナトリウム、pH 7.2、10 mg/mL ウシ血清アルブミン（BSA）-免疫グロブリン含有（プロテアーゼフリー）

再構成

Reconstitute with 500 μ;L deionized water (or equivalent).

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• ピクトグラム: GHS06,GHS09
• シグナルワード: Danger
• 危険有害性情報: H302,H311,H411
• 注意書き: P280 - P301 + P312 + P330 - P302 + P352 + P312
• 危険有害性の分類: Acute Tox. 3 Dermal - Acute Tox. 4 Oral - Aquatic Chronic 2
• 補足的ハザード: EUH032
• 保管分類コード: 6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
• WGK: WGK 3
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable