SAB3700670

Anti-Rat IgM (μ-chain specific) antibody produced in goat

affinity isolated antibody, buffered aqueous solution

• UNSPSCコード: 12352203
• NACRES: NA.46

特性

• 由来生物: goat
• 結合体: unconjugated
• 抗体製品の状態: affinity isolated antibody
• 抗体製品タイプ: secondary antibodies
• クローン: polyclonal
• 形状: buffered aqueous solution
• 化学種の反応性: rat
• 濃度: 1.0 mg/mL
• テクニック: ELISA: suitable; immunohistochemistry: suitable; western blot: suitable
• 輸送温度: wet ice
• 保管温度: 2-8°C
• ターゲットの翻訳後修飾: unmodified

詳細

詳細

Immunoglobulin M (IgM) belongs to the immunoglobulin family. It exists as a pentamer in the serum and has a high molecular weight. Monomeric IgM has a molecular weight of 190kDa. The pentameric IgM consists of ten μ-heavy chains, ten light chains and a single J-chain which are linked by disulphide bonds. The μ-chain consists of five domains- VH, Cμ1, Cμ2, Cμ3 and Cμ4. Limited digestion using papain cleaves the antibody into three fragments, two of which are identical and contain the antigen-binding activity. They are known as Fragment antigen binding (Fab) fragments. The third fragment does not possess antigen-binding activity and is known as fragment crystallizable (Fc). It interacts with cells and effector molecules. The heavy chains of IgM contain an extra C domain that replaces the hinge region present between Fab and Fc. IgM is the first antibody to be produced during an immune response and is the major isotype secreted in T-cell independent immune responses.

特異性

This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgM coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against Anti-Goat Serum, Rat IgM and Rat Serum. No reaction was observed against Rat IgG or Rat IgA. Specificity was confirmed by ELISA at less than 1% cross reactivity against other rat heavy or light chain isotypes.

免疫原

Rat IgM mu heavy chain

物性

抗体フォーマット：IgG

物理的形状

0.02 M リン酸カリウム、0.15 M 塩化ナトリウム、pH 7.2

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 10 - Combustible liquids
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable