S5933

抗STAT3抗体 ウサギ宿主抗体

IgG fraction of antiserum, buffered aqueous solution

• MDL番号: MFCD01092492
• UNSPSCコード: 12352203
• NACRES: NA.46

特性

• 由来生物: rabbit
• 品質水準: 200
• 結合体: unconjugated
• 抗体製品の状態: IgG fraction of antiserum
• 抗体製品タイプ: primary antibodies
• クローン: polyclonal
• 形状: buffered aqueous solution
• 分子量: antigen 92 kDa
• 化学種の反応性: human, rat, mouse
• テクニック: immunocytochemistry: 10 μg/mL using A431 cells; immunoprecipitation (IP): 4 μg using STAT3 from 0.5–1 mg of an EGF stimulated human A431 cell lysate; western blot (chemiluminescent): 0.5-2 μg/mL using lysates from EGF stimulated human A431, mouse WEHI and rat L6 cells
• UniProtアクセッション番号: P40763
• 輸送温度: dry ice
• 保管温度: −20°C
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... STAT3(6774) ; mouse ... Stat3(20848) ; rat ... Stat3(25125)

詳細

詳細

Signal transducer and activator of transcription 3 (STAT3) protein is a member of the STAT family of proteins. It consists of a DNA binding domain, a Src homology-2 (SH-2) domain, an N-terminal coiled-coiled domain, and a C-terminal transactivation domain. The STAT3 gene is located on the human chromosome at 17q21.2.

特異性

STAT3に特異的な抗体です。高濃度で長時間反応させると、さらに66kDaのバンドを検出する場合があります。 本抗体の認識配列(688-722)は、マウス、ラットSTAT3と一致します。最初の28個のアミノ酸はマウスSTAT3Bと同一です。

免疫原

バクテリアで発現した、ヒトSTAT3のアミノ酸688~722に相当するGST融合タンパク質。

アプリケーション

Anti-STAT3 antibody produced in rabbit has been used in western blotting.

生物化学的／生理学的作用

Signal transducer and activator of transcription 3 (STAT3) signaling participates in cancer inflammation. This protein interacts with nuclear factor-κB (NF-κB). STAT3 regulates the function of several genes that take part in proliferation, apoptosis, metastasis, angiogenesis, immune response, and cell differentiation. Overexpression of the STAT3 gene is associated with lymphomas, prostate carcinoma, breast cancer, hepatocellular carcinoma, pancreatic adenocarcinoma, cholangiocarcinoma, and ovarian carcinoma.

物理的形状

0.1 Mトリス-グリシン溶液(pH 7.4, 0.15M NaCl, 0.05%アジ化ナトリウム含有)。

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 10 - Combustible liquids
• WGK: nwg
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable