R8529

Monoclonal Anti-RAL-A antibody produced in mouse

~2 mg/mL, clone RalA5F9.2, purified immunoglobulin, buffered aqueous solution

• MDL番号: MFCD08705597
• UNSPSCコード: 12352203
• NACRES: NA.41

特性

• 由来生物: mouse
• 品質水準: 200
• 結合体: unconjugated
• 抗体製品の状態: purified immunoglobulin
• 抗体製品タイプ: primary antibodies
• クローン: RalA5F9.2, monoclonal
• 形状: buffered aqueous solution
• 分子量: antigen 25 kDa
• 化学種の反応性: monkey, mouse, human, rat
• 濃度: ~2 mg/mL
• テクニック: immunocytochemistry: 5-10 μg/mL using HeLa cells; indirect ELISA: suitable; microarray: suitable; western blot: 1-2 μg/mL using rat brain extract
• アイソタイプ: IgG1
• UniProtアクセッション番号: P11233
• 輸送温度: dry ice
• 保管温度: −20°C
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... RALA(5898) ; mouse ... Rala(56044) ; rat ... Rala(81757)

詳細

詳細

Monoclonal Anti-Ral-A (mouse IgG1 isotype) is derived from the hybridoma RalA5F9.2 produced by the fusion of mouse myeloma cells (NS1 cells) and splenocytes from BALB/c mice immunized with a synthetic peptide. Ras-related protein Ral-A is a member of the Ras-like guanosine-5′-triphosphate (GTP) binding protein family. Ral-A protein is expressed in the islets of the pancreas, insulin-secreting cell lines and tissues during embryogenesis. The RALA gene is located on the human chromosome at 7p14.1.

特異性

Monoclonal Anti-Ral-A recognizes human, monkey, rat and mouse Ral-A, ~25 kDa.

免疫原

synthetic peptide corresponding to amino-acids 189-203 of human Ral-A, conjugated to KLH.

アプリケーション

Monoclonal Anti-RAL-A antibody produced in mouse may be used in:

• immunoblotting
• immunocytochemistry
• enzyme-linked immunosorbent assay (ELISA)

生物化学的／生理学的作用

Ras-related protein Ral-A can bind guanosine triphosphate (GTP) or guanosine diphosphate (GDP). It is known to possess a low intrinsic GTPase activity. Ral-A protein is involved in regulating the insulin secretion of pancreatic β-cells. This protein is also involved in membrane trafficking, oncogenic transformation and proliferation.

物理的形状

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

保管および安定性

For extended storage, freeze at –20 °C in working aliquots. For continuous use, store at 2-8 °C for up to one month. Repeated freezing and thawing, or storage in "frost-free" freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

免責事項

Unless otherwise stated in our catalog, our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 10 - Combustible liquids
• WGK: WGK 3
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable
• 個人用保護具 (PPE): Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)