R1656

RAF1(EE)(306-末端)、活性型、GSTタグ融合 ヒト

PRECISIO^® Kinase, recombinant, expressed in baculovirus infected Sf9 cells, ≥70% (SDS-PAGE), buffered aqueous glycerol solution

• 別名: CMD1NN, NS5, RAF proto-oncogene serine/threonine-protein kinase, cRaf
• UNSPSCコード: 12352200
• NACRES: NA.32

特性

• 由来生物: fermentation/recombinant
• 品質水準: 200
• リコンビナント: expressed in baculovirus infected Sf9 cells
• 製品種目: PRECISIO^® Kinase
• アッセイ: ≥70% (SDS-PAGE)
• フォーム: buffered aqueous glycerol solution
• 比活性: 5100-6900 nmol/min·mg
• 分子量: ~63 kDa
• メーカー／製品名: Precision
• テクニック: cell based assay: suitable
• 溶解性: water: soluble
• タンパク質IDアクセッション番号: NM 002880
• UniProtアクセッション番号: P04049
• 輸送温度: dry ice
• 保管温度: −70°C
• 遺伝子情報: human ... RAF1(5894)

詳細

詳細

Research area: Cell Signaling

Rapidly Accelerated Fibrosarcoma (RAF) belongs to receptor tyrosine kinase (RTK) family. RAF exists as three isoforms A, B and C-RAF or RAF-1. RAF1 links Ras family members with mitogen-activated protein kinase/extracellular signal-regulated kinase (MAPK/ERK) signaling cascade. The RAF-1 gene is mapped to the human chromosome at 3p25.2.

生物化学的／生理学的作用

Rapidly Accelerated Fibrosarcoma 1(RAF1), a member of MAP kinase kinase kinase (MAP3K) family plays a vital role in cell signaling by directly interacting with GTPases and functions downstream in their signaling pathway. RaF1 is activated by Ras in the presence of growth-promoting signals, leading to the activation of mitogen-activated protein kinase kinase (MEK1/MEK2) and extracellular signal-regulated protein kinases 1 and 2 (ERK1/ERK2) downstream. The activated ERK proteins control gene expression related to cell division, apoptosis, cell differentiation, and cell migration. The gene is mutated or upregulated in prostate and bladder cancer. Also, mutations are associated with Noonan syndrome, which leads to various physical abnormalities like craniofacial dysmorphisms, ocular, and musculoskeletal irregularities, neurocognitive impairment, and cardiac malformations.

物理的形状

50 mM Tris-HCl溶液(pH 7.5、150 mM NaCl、0.25 mM DTT、0.1 mM EGTA、0.1 mM EDTA、0.1 mM PMSF、25%グリセロール含有)。

法的情報

PRECISIO is a registered trademark of Merck KGaA, Darmstadt, Germany

安全性情報

• 保管分類コード: 10 - Combustible liquids
• WGK: WGK 1
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable
• 個人用保護具 (PPE): Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)