P6246

モノクロナール抗PABP マウス宿主抗体

clone 10E10, purified from hybridoma cell culture

• 別名: 抗ポリ(A)結合タンパク質抗体
• MDL番号: MFCD06412253
• UNSPSCコード: 12352203
• NACRES: NA.41

特性

• 由来生物: mouse
• 結合体: unconjugated
• 抗体製品の状態: purified from hybridoma cell culture
• 抗体製品タイプ: primary antibodies
• クローン: 10E10, monoclonal
• フォーム: buffered aqueous solution
• 分子量: antigen ~69 kDa
• 交差性: hamster, canine, bovine, Xenopus, human, monkey
• テクニック: immunocytochemistry: suitable; immunoprecipitation (IP): suitable; microarray: suitable; western blot: 0.5-1 μg/mL using whole cell extracts of HEK 293T cells
• アイソタイプ: IgG1
• UniProtアクセッション番号: P11940
• 輸送温度: dry ice
• 保管温度: −20°C
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... PABPC1(26986)

詳細

詳細

Monoclonal Anti-PABP (mouse IgG1 isotype) is derived from the 10E10 hybridoma produced by the fusion of mouse myeloma cells (SP2/0) and splenocytes from BALB/c mice immunized with recombinant human PABP. Poly(A) binding protein (PABP) is highly conserved between different organisms especially at the amino-terminal region of the protein that contains four RNA-binding domains (RBDs).

Poly(A) binding protein (PABP) possesses RNA recognition motifs (RRMs), a proline-rich region and a PABP C-terminal domain. The gene encoding this protein is localized on human chromosome 8q22.3.

免疫原

リコンビナントヒトPABP。

アプリケーション

Monoclonal Anti-PABP antibody produced in mouse has been used for immunofluorescence.

Monoclonal Anti-PABP antibody produced in mouse has been used in:

• Immunoblotting
• Immunostaining
• Immunoprecipitation
• Enzyme linked immunosorbent assay(ELISA)
• Immunocytochemistry

生物化学的／生理学的作用

Poly(A) binding protein (PABP) has a role in mRNA translation and stability. It modulates gene expression. The protein binds to the poly(A) tail, bridges the ends of mRNA and forms a closed loop. This might help in translation initiation by promoting ribosome recruitment.

Poly(A) binding protein (PABP) is found in excess, relative to the amount of cytoplasmic poly(A) (three fold) and binds to its poly(A) target at high affinity (Kd of 7nM). The eukaryotic initiation factor, EIF4F complex, interacts with PABP indirectly via the PAIP-1 protein (PABP-interacting protein-1). As a consequence, an interaction between the 5′ and 3′ ends of mRNA occurs. This proximity between the mRNA ends contributes to its stability and enhances translation, since terminating ribosomes may start translation again.

物理的形状

0.01M PBS溶液 (pH 7.4, 15mMアジ化ナトリウム含有)。

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 10 - Combustible liquids
• WGK: nwg
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable