P4749

Anti-PADI4 (N-terminal) antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

• 別名: 抗PADI5抗体, 抗PDI4抗体
• MDL番号: MFCD09039202
• UNSPSCコード: 12352203
• NACRES: NA.41

特性

• 由来生物: rabbit
• 品質水準: 200
• 結合体: unconjugated
• 抗体製品の状態: IgG fraction of antiserum
• 抗体製品タイプ: primary antibodies
• クローン: polyclonal
• フォーム: buffered aqueous solution
• 分子量: antigen 67 kDa
• 化学種の反応性: human
• 強化検証: recombinant expression; Learn more about Antibody Enhanced Validation
• テクニック: immunoprecipitation (IP): 2-4 μL using DMSO-treated HL60 cells; microarray: suitable; western blot (chemiluminescent): 1:500-1:1,000 using DMSO-treated HL60 cells
• UniProtアクセッション番号: Q9UM07
• 輸送温度: dry ice
• 保管温度: −20°C
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... PADI4(23569)

詳細

詳細

PAD4 (also designated PADI4 or PAD type IV), one of the members of the PAD family. PAD4 was initially named PAD5. However, since it is closely related to rodent PAD4 it was renamed human PAD4. The most noticeable difference between the isotypes is their tissue-specific expression. Expression of the PAD4 gene is tightly linked to myeloid differentiation. It is expressed in granulocytes and monocytes and expressed in the nucleus.

Peptidyl arginine deiminase 4 (PADI4) is one of the four PADIs found in humans. The protein consists of 663 amino acids. PADI4 gene is localized to human chromosome 1p36. It is expressed in hematopoietic tissues, such as spleen, thymus, peripheral blood leucocytes, fetal liver and bone marrow.

免疫原

synthetic peptide corresponding to amino acids 1-15 of human PADI4, conjugated to KLH via a C-terminal added cysteine residue. The immunizing peptide is specific to human.

アプリケーション

Anti-PADI4 (N-terminal) antibody produced in rabbit has been used in immunoblotting and immunoprecipitation.

Anti-PADI4 (N-terminal) antibody produced in rabbit has been used in immunohistochemistry and immunofluorescence.

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Chromatin immunoprecipitation (1 paper)

生物化学的／生理学的作用

PADs (also known as PADIs), peptidylarginine deiminases, are a family of enzymes, which convert protein-bound arginine residues to citrulline residues in the presence of calcium ions, a process termed deimination. Among the 5 isoforms of PADs, only PAD4 has been identified as an RA-susceptibility gene.

Peptidyl arginine deiminase 4 (PADI4) is responsible for the post-translational conversion of peptidylarginine to citrulline, in the presence of calcium ions. In individuals with rheumatoid arthritis (RA), this gene is expressed in hematological cells and synovial tissues, and variant in this gene is linked with susceptibility to RA. The expression of this protein is linked with DNA hypermethylation in acute promyelocytic leukemia (APL).

物理的形状

Solution in 0.01 M phosphate buffered saline, pH 7.4, and 15 mM sodium azide.

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 10 - Combustible liquids
• WGK: WGK 3
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable