由来生物
mouse
結合体
unconjugated
抗体製品の状態
ascites fluid
抗体製品タイプ
primary antibodies
クローン
PKB-175, monoclonal
分子量
antigen 56 kDa
含みます
15 mM sodium azide
化学種の反応性
chicken, human, bovine, rat, mouse
テクニック
indirect ELISA: suitable
microarray: suitable
western blot: 1:5,000 using human MCF-7 cell extract
アイソタイプ
IgG1
UniProtアクセッション番号
輸送温度
dry ice
保管温度
−20°C
ターゲットの翻訳後修飾
unmodified
遺伝子情報
human ... AKT1(207)
mouse ... Akt1(11651)
rat ... Akt1(24185)
詳細
Monoclonal Anti-Protein Kinase Bα (PKBα/Akt1) (mouse IgG1 isotype) is derived from the PKB-175 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from a BALB/c mouse. PKB/Akt is composed of an N-terminal pleckstrin-homology (PH) domain, followed by a catalytic kinase domain and a short C-terminal regulatory domain. This cytoplasmic protein is made up of 480 amino acids. AKT1 is mapped to human chromosome 14q32.
Protein Kinase B (also known as Akt, or RAC-PK) is a protein that belongs to serine/threonine kinases.
特異性
Monoclonal Anti-Protein Kinase Bα (PKBα/Akt1) recognizes PKB isoform α.
免疫原
Synthetic peptide corresponding to amino acids 461-477 of human PKBα/Akt1, conjugated to KLH. The sequence is identical in mouse, rat, and bovine PKBα/Akt1, highly conserved in PKBβ/Akt2, and diverges in PKBγ.
アプリケーション
Monoclonal Anti-Protein Kinase Bα antibody produced in mouse has been used in:
- western blot
- immunofluorescence
- enzyme-linked immunosorbent assay (ELISA)
生物化学的／生理学的作用
Protein kinase Bα (PKBα/Akt1) plays a crucial role as a suppressor of apoptotic cell death in different cell types, induced by a variety of stimuli including growth factor withdrawal, loss of cell adhesion and DNA damage. It can protect cerebellar neurons from apoptosis induced by insulin-like growth factor 1 (IGF-1) withdrawal. Akt is the cellular homologue of the viral oncogene v-akt of the AKT-8 acute transforming retrovirus found in rodent T cell lymphoma. It is considered to play an important role in the control of cell cycle, cell proliferation and differentiation . AKT plays a key role in angiogenesis.
物理的形状
Monoclonal Anti-Protein Kinase Bα (PKBα/Akt1) is supplied as ascites fluid containing 15 mM sodium azide
保管および安定性
For continuous use, store at 2-8C for upto one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing is not recommended. Storage in "frost-free" freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12hours.
免責事項
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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保管分類コード
12 - Non Combustible Liquids
WGK
nwg
引火点(°F)
Not applicable
引火点(℃)
Not applicable
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
Jan Code
P2482-.2ML:
P2482-BULK:
P2482-VAR:
試験成績書（COA）
製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
Activation of Akt pathway by transcription-independent mechanisms of retinoic acid promotes survival and invasion in lung cancer cells
Garcia RA, et al.
Molecular Cancer, 12(1), 44-44 (2013)
Shuyi Nie et al.
Mechanisms of development, 124(9-10), 657-667 (2007-08-25)
ErbB signaling regulates cell adhesion and movements during Xenopus gastrulation, but the downstream pathways involved have not been elucidated. In this study, we show that phosphatidylinositol-3 kinase (PI3K) and Erk mitogen-activated protein kinase (MAPK) mediate ErbB signaling to regulate gastrulation.
Jinling Wu et al.
Development (Cambridge, England), 133(18), 3651-3660 (2006-08-18)
GIPC is a PDZ-domain-containing protein identified in vertebrate and invertebrate organisms through its interaction with a variety of binding partners including many membrane proteins. Despite the multiple reports identifying GIPC, its endogenous function and the physiological significance of these interactions
Rodolfo Daniel Cervantes-Villagrana et al.
The Journal of biological chemistry, 294(2), 531-546 (2018-11-18)
G protein-coupled receptors stimulate Rho guanine nucleotide exchange factors that promote mammalian cell migration. Rac and Rho GTPases exert opposing effects on cell morphology and are stimulated downstream of Gβγ and Gα12/13 or Gαq, respectively. These Gα subunits might in
P J Coffer et al.
European journal of biochemistry, 201(2), 475-481 (1991-10-15)
Highly degenerate oligonucleotide primers designed from regions conserved between protein-serine kinases have been used specifically to amplify human epithelial (HeLa) cDNA by the polymerase chain reaction (PCR). Of several novel cDNA fragments encoding putative kinases thus isolated, one was further
資料
Quantitative and qualitative western blotting to validate knockdown by esiRNA. Sigma-Aldrich.com
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