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由来生物
rabbit
結合体
unconjugated
抗体製品の状態
IgG fraction of antiserum
抗体製品タイプ
primary antibodies
クローン
polyclonal
形状
buffered aqueous solution
分子量
antigen 150-160 kDa
化学種の反応性
rat
テクニック
western blot: 1:10,000 using rat brain extract
UniProtアクセッション番号
輸送温度
dry ice
保管温度
−20°C
ターゲットの翻訳後修飾
unmodified
遺伝子情報
human ... NOS1(4842)
rat ... Nos1(24598)
詳細
In humans, nitric oxide synthase 1 (NOS1) or neuronal nitric acid synthase (nNOS) is localized to the sarcoplasmic reticulum. The gene encoding it is, localized on human chromosome 12q24.22. In rats, the protein is present in perivascular nerves supplying the cerebral vasculature. The gene encoding it is localized on rat chromosome 12q16.
The nitric oxide synthase (NOS) isoform found in neurons is a 150-160 kDa protein. It is also termed brain NOS, NOS1, neuronal NOS (nNOS or bNOS, neuronal constitutive NOS or Ca2+-regulated NOS (cNOS, ncNOS). The nNOS or NOS1 gene is mapped to human chromosome 12q24.22. bNOS is present in skeletal muscle, central, peripheral neurons and neuronal cell bodies. Human bNOS and endothelial nitric oxide synthase (eNOS) share 52% amino acid identity, and rat and human bNOS share 93% amino acid identity. Structurally NOS exists as homodimers. The N-terminal region comprises heme and calmodulin binding sites. The reduced nicotinamide adenine dinucleotide phosphate (NADPH) and flavins binding regions are localized in C-terminal region.
特異性
The antibody is specific for nitric oxide synthase (NOS) derived from brain. It does not recognize NOS derived from activated macrophages (iNOS) and endothelial cells (eNOS).
免疫原
ラット脳由来の一酸化窒素合成酵素(NOS)(bNOS、アミノ酸1409~1429番目) に相当する合成ペプチドで、KLHにコンジュゲ-トされています。この免疫原の配列は、ヒト bNOS とはアミノ酸残基１個だけ異なります。
アプリケーション
Anti-Nitric Oxide Synthase, Brain (1409-1429) antibody produced in rabbit has been used in:
- immunoblotting
- immunostaining
- immunohistochemistry
- immunofluorescence
- immunocytochemistry
生物化学的／生理学的作用
Nitric oxide synthase (NOS), brain (nNOS or bNOS) mediates long-term regulation of synaptic transmission (long-term potentiation, long-term inhibition). It catalyzes the production of nitric oxide (NO) from L-arginine. The NO mediates neurovascular coupling and is associated with neuronal plasticity and pain signal transmission. nNOS regulates blood pressure, relaxation of smooth muscle and vasodilatation via peripheral nitrergic nerves. nNOS is complexed to dystrophin. However, in Duchenne muscular dystrophy (DMD), the skeletal muscle sarcolemma is devoid of nNOS.
物理的形状
15 mMアジ化ナトリウム含有のpH 7.4の0.01 Mリン酸緩衝食塩水溶液
保管および安定性
Store at 2-8 °C for up to one month. For extended storage, solution may be frozen in working aliquots. Repeated freezing and thawing, or storage in "frost-free" freezers,is not recommended. If slight turbidity occurs upon prolonged storage, clarify by centrifugation before use.
免責事項
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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保管分類コード
10 - Combustible liquids
WGK
WGK 3
引火点(°F)
Not applicable
引火点(℃)
Not applicable
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
Jan Code
N7280-VAR:
N7280-.2ML:
N7280-BULK:
IXO11098:
試験成績書（COA）
製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
Selective synaptic cadherin expression by traced neurons of the chicken visual system
Dominik Heyers
The Journal of Neuroscience (2004)
Kristi A Kohlmeier et al.
Frontiers in neuroscience, 7, 246-246 (2014-01-07)
Orexin neuropeptides influence multiple homeostatic functions and play an essential role in the expression of normal sleep-wake behavior. While their two known receptors (OX1 and OX2) are targets for novel pharmacotherapeutics, the actions mediated by each receptor remain largely unexplored.
Integrated genome-wide association study findings: identification of a neurodevelopmental network for attention deficit hyperactivity disorder
Geert Poelmans
The American Journal of Psychiatry (2011)
Michael Leist et al.
Scientific reports, 6, 24904-24904 (2016-04-29)
Although hyperpolarization-activated cyclic nucleotide-gated cation (HCN) channels and the corresponding h-current (Ih) have been shown to fundamentally shape the activity pattern in the thalamocortical network, little is known about their function in local circuit GABAergic interneurons (IN) of the dorsal
Kinga Gzielo et al.
Cellular and molecular neurobiology, 37(5), 783-789 (2016-08-20)
Obesity in humans is associated with cognitive decline and elevated risk of neurodegenerative diseases of old age. Variations of high-fat diet are often used to model these effects in animal studies. However, we previously reported improvements in markers of memory
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