N6288

Anti-NucleolinAtto 488 ウサギ宿主抗体

1.5-3.0 mg/mL, affinity isolated antibody, buffered aqueous solution

• 別名: Anti-NCL, PROTEIN C23
• UNSPSCコード: 12352203
• NACRES: NA.41

特性

• 由来生物: rabbit
• 結合体: Atto 488 conjugate
• 抗体製品の状態: affinity isolated antibody
• 抗体製品タイプ: primary antibodies
• クローン: polyclonal
• フォーム: buffered aqueous solution
• 交差性: human, mouse, rat
• 保管条件: protect from light
• 濃度: 1.5-3.0 mg/mL
• テクニック: direct immunofluorescence: 0.5-1.0 μg/mL using human HeLa, rat NRK and mouse 3T3 cells
• 蛍光検出: λ_ex 500 nm; λ_em 522 nm in PBS; λ_ex 500 nm; λ_em 522 nm
• UniProtアクセッション番号: P19338
• 輸送温度: dry ice
• 保管温度: −20°C
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... NCL(4691) ; mouse ... Ncl(17975) ; rat ... Ncl(25135)

詳細

詳細

詳しくは こちらをご覧ください。

Nucleolin is a multi-domain phosphoprotein mapped to human chromosome 2q37. It comprises three domains and a bipartite nuclear localization signal sequence. Nucleolin domains include the highly acidic N-terminal region, a RNA-binding central domain and a glycine, arginine and phenylalanine residues rich C-terminal region.

特異性

Anti-Nucleolin-Atto 488 recognizes human, rat, and mouse nucleolin.

免疫原

synthetic peptide corresponding to amino acid residues 2-17 of human nucleolin conjugated to KLH. The corresponding sequence differs by 4 amino acids in rat and mouse nucleolin, and by one amino acid in the rat homolog protein NRP (nucleolin related protein).

アプリケーション

Anti-Nucleolin−Atto 488 antibody produced in rabbit has been used in immunocytochemistry and immunofluorescence.

生物化学的／生理学的作用

Nucleolin (NCL) interacts with proteins participating in microtubule nucleation. It takes part in the transcription of polymerase I, formation of ribosomes and duplication of centrosomes. NCL is also a transcription regulator. Studies have shown that it is highly expressed in many tumors.

Nucleolin N- and C-terminal domains are involved in protein-protein interaction, whereas the central domain is involved in specific interactions with nucleic acids. Nucleolin exhibits autodegradation, DNA and RNA helicase activities, and DNA-dependent ATPase activity. Nucleolin′s multiple activities may be regulated by proteolysis and methylation. It also interacts with telomerase andthis interaction is critical for the nucleolar localization of telomerase. Nucleolin might function as a cell surface receptor for lipoproteins, viruses, extracellular matrix, growth factors and other molecules.

物理的形状

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

保管および安定性

For continuous use, store at 2-8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 10 - Combustible liquids
• WGK: WGK 2
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable
• 個人用保護具 (PPE): Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)