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製品名
OATP1B3 Knockout HepaRG™ Cells, 1 vial
由来生物
human female liver (hepatocarcinoma and hepatitis C tumor)
品質水準
フォーム
liquid
OMIMアクセッション番号
保管温度
−196°C
遺伝子情報
human ... OATP1B3(28234)
詳細
HepaRG is a human hepatoma cell line. The cells possess a pseudodiploid karyotype and have been characterized as an oval ductular bipotent hepatic cell line as they have the ability to differentiate into both biliary and hepatocyte lineages in the presence of DMSO. HepaRG OATP1B3 knockout cells express the major xenobiotic sensors (PXR, CAR and AhR), drug transporters, phase I and II drug metabolizing enzymes as well as key hepatic transcription factors involved in stress response pathways.
アプリケーション
See technical bulletin for detailed protocols
特徴および利点
Zinc finger nucleases (ZFN) mediated Knock-out of SLCO1B3 (MRP3) gene.
- The frame-shift mutation of SLCO1B3 gene was confirmed by fragment length analysis and DNA sequencing.
- Loss of functionality was confirmed by loss of transport of selective substrates in sandwich culture assay.
品質
Tested for Mycoplasma, sterility, post-freeze viability, short terminal repeat (STR) analysis for cell line identification, cytochrome oxidase I (COI) analysis for cell line species confirmation.
法的情報
These products are covered by the License Agreement as described in Exhibit 1, 2 and 3, in the technical bulletin.
HepaRG is a trademark of BioPredic International company
免責事項
RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.
保管分類コード
12 - Non Combustible Liquids
WGK
WGK 3
引火点(°F)
Not applicable
引火点(℃)
Not applicable
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
Jan Code
MTOX1016-1VL:
最新バージョンのいずれかを選択してください：
資料
Oral drug delivery involves dissolution in the small intestine and absorption across the enterocyte barrier into the portal vein followed by subsequent delivery through the liver into the systemic circulation.
Sigma-Aldrich presents an article on The Role of Liver Transporters in Drug-Drug Interactions
ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.
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