MAK359

Mitochondrial Complex I Activity Assay Kit

sufficient for 100 colorimetric tests

• UNSPSCコード: 12352200

特性

• 使用法: sufficient for 100 colorimetric tests
• 検出方法: colorimetric

詳細

詳細

Mitochondrial Complex I or NADH:ubiquinone oxidoreductase is the first and the largest complex of the Electron Transport Chain located in the mitochondrial membrane. It oxidizes NADH to NAD+ and transfers an electron to ubiquinone (also present in the inner mitochondrial membrane) converting it to ubiquinol. During this process, it transports protons across the inner mitochondrial membrane, helping to develop an electrochemical gradient. This process is very important for cellular respiration and adverse effects on Complex I activity can compromise mitochondrial respiration, which further leads to cellular stress.

適合性

Suitable for the measurement of Mitochondrial Complex I activity in isolated mitochondria.

原理

The Mitochondrial Complex I Assay Kit is a fast and reliable method to determine the activity of complex I in isolated mitochondria. It is useful for respiration studies in isolated mitochondria and may be used to study effects of toxicants, drugs and other environmental conditions on mitochondrial complex I activity. This kit uses decylubiquinone, an analog of ubiquinone, as an electron acceptor that gets converted to decylubiquinol through the catalytic activity of Complex I. The Complex I dye that absorbs light at A600 nm in its oxidized form is used as a terminal electron acceptor that accepts electrons from decylubiquinol. Complex I activity is determined colorimetrically by recording the change in absorbance of reduced Complex I dye at A600 nm. Specific Complex I activity is obtained by subtracting the activity in presence of Complex I inhibitor rotenone from total activity. This kit can detect as low as 0.1 mU / well and is linear up to 7 mU / well.

安全性情報

• 危険有害性情報: H412
• 注意書き: P273 - P501
• 危険有害性の分類: Aquatic Chronic 3
• 保管分類コード: 10 - Combustible liquids
• 引火点(°F): 188.6 °F
• 引火点(℃): 87 °C