M5437

Anti-MSK-1 antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

• MDL番号: MFCD02686432
• UNSPSCコード: 12352203
• NACRES: NA.44
• 結合体: unconjugated
• application: ARR; ICC; WB
• クローン: polyclonal
• 化学種の反応性: rat
• citations: 9
• テクニック: immunocytochemistry: 1:1,000 using methanol/acetone-fixed rat fibroblast Rat-1 cell line; microarray: suitable; western blot: 1:2,000 using using whole cell lysate of rat fibroblast Rat-1 cell line

特性

• 由来生物: rabbit
• 品質水準: 200
• 結合体: unconjugated
• 抗体製品の状態: IgG fraction of antiserum
• 抗体製品タイプ: primary antibodies
• クローン: polyclonal
• フォーム: buffered aqueous solution
• 分子量: antigen 90 kDa
• 化学種の反応性: rat
• テクニック: immunocytochemistry: 1:1,000 using methanol/acetone-fixed rat fibroblast Rat-1 cell line; microarray: suitable; western blot: 1:2,000 using using whole cell lysate of rat fibroblast Rat-1 cell line
• 輸送温度: dry ice
• 保管温度: −20°C
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... RPS6KA5(9252) ; rat ... Rps6ka5(314384)

詳細

詳細

The mitogen- and stress-activated kinases, MSK-1 and MSK-2 are activated by ERK/MAPK and p38 MAPK in response to growth and cell stress stimuli. MSK-1 and MSK-2 have 75% homology and interact with ERK1/2 and p38 through MAPK-binding domains near the C-terminus. There is a predominant expression of MSK proteins in heart, brain, skeletal muscles, lung, liver, kidney, pancreas and placenta. Highly conserved phosphorylation sites of MSK-1, Ser260 and Thr581, are followed by proline residues indicating that MSK-1 is activated by more than one MAPK. Endogenous MSK-1 is activated in cells by growth factors, phorbol ester stimulation, exposure to UV irradiation, and oxidative or chemical stress and is localized to nucleus of unstimulated or stimulated cells. MSK-1 is directly involved in the regulation of gene expression since it actively phosphorylates, stabilizes and activates many transcription factors. The major transcription factors that are regulated by MSK-1 include CREB, NF-κB and STAT3. MSKs also evidently phosphorylate Histone H3 at Ser10 and Ser28 and HMG at Ser6. Thus a primary role of MSK proteins is also promotion of gene relaxation and activation by regulating the binding of transcription repressors. Anti-MSK-1 specifically recognizes rat MSK-1 (90 kDa).

免疫原

ヒトMSK1のC末端に相当する合成ペプチド(アミノ酸783-800)。この配列はヒト, マウスMSK2アイソフォームには存在しません。

アプリケーション

Anti-MSK-1 may be used at a minimum working dilution of 1:2000 (immunoblotting in whole cell extracts) or 1:1000 (immunocytochemistry) in fibroblast Rat1 cell line. The antobody has also been used for detection by immunoblotting in mouse fibroblast 10T1/2 cells.

物理的形状

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 10 - Combustible liquids
• WGK: WGK 3
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable