M042030

ビオチン ホスホロアミダイト

configured for ABI

• 別名: Biotin amidite
• 実験式（ヒル表記法）: C₄₆H₆₄N₅O₈PS
• CAS番号: 1217500-22-5
• 分子量: 878.07
• MDL番号: MFCD00676535
• UNSPSCコード: 41116105
• PubChem Substance ID: 329817780
• NACRES: NA.21

特性

• 製品種目: Proligo Reagents
• 品質水準: 200
• アッセイ: ≥96% (³¹P-NMR)
• 適合性: conforms to structure for H-NMR; configured for ABI
• 保管温度: −20°C
• SMILES記法: COc1ccc(cc1)C(N2C3CS[C@@H](CCCCC(=O)NCCOCCOCCOP(OCCC#N)N(C(C)C)C(C)C)C3NC2=O)(c4ccccc4)c5ccc(OC)cc5
• InChI: 1S/C46H64N5O8PS/c1-34(2)51(35(3)4)60(58-27-12-25-47)59-32-31-57-30-29-56-28-26-48-43(52)16-11-10-15-42-44-41(33-61-42)50(45(53)49-44)46(36-13-8-7-9-14-36,37-17-21-39(54-5)22-18-37)38-19-23-40(55-6)24-20-38/h7-9,13-14,17-24,34-35,41-42,44H,10-12,15-16,26-33H2,1-6H3,(H,48,52)(H,49,53)/t41-,42-,44-,60?/m0/s1
• InChI Key: WSDQYLGSIUJJRU-ZMKGMYJVSA-N

詳細

詳細

Biotin phosphoramidite is a reagent used to directly incorporate a biotin label at the 5′ hydroxy group of the terminal nucleoside base through a p-aminophenethyl spacer group during oligonucleotide synthesis. Biotin-labelled oligonucleotides have become extremely popular in DNAcapture and detection applications, due to their very high-sensitivity and strong, specific binding affinity with avidin and streptavidin proteins. Biotinbased detection methods and are widely used by researchers. Biotin labelling is compatible with PCR and most hybridization techniques.

Key Features of Biotin Phosphoramidite

• Behaves like any standard phosphoramidite on the DNA synthesizer
• Readily soluble in acetonitrile
• Biotin compound has a dimethoxytrityl (DMT) group on the ring structure,which allows coupling-yield determination and trityl-selective purification
• A long, water-compatible linker arm ensures maximum sensitivity
• Provides a coupling efficiency of at least 95%The product is configured for ABI^™ Synthesizers

法的情報

ABI is a trademark of Applera Corporation or its subsidiaries in the US and/or certain other countries

安全性情報

• 保管分類コード: 11 - Combustible Solids
• WGK: WGK 3
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable