由来生物
rabbit
結合体
unconjugated
抗体製品の状態
IgG fraction of antiserum
抗体製品タイプ
primary antibodies
クローン
polyclonal
形状
buffered aqueous solution
化学種の反応性
human, mouse, rat
テクニック
microarray: suitable
western blot: 1:2,000 using a cytosolic fraction of rat brain.
UniProtアクセッション番号
輸送温度
dry ice
保管温度
−20°C
ターゲットの翻訳後修飾
unmodified
遺伝子情報
human ... LIMK1(3984)
mouse ... Limk1(16885)
rat ... Limk1(65172)
詳細
LIM Domain Kinase 1 (LIMK1) belongs to the LIMK family of serine/threonine protein kinases. LIMK1 shares about 50% sequence identity with LIMK2. LIMK1 contains two N-terminal LIM domains, an internal PDZ (post synaptic density protein (PSD95), Drosophila disc large tumor suppressor (Dlg1), and zonula occludens-1 protein (zo-1)) domain and a C-terminal kinase domain.
免疫原
Synthetic peptide corresponding to the C-terminus of mouse LIMK1 (amino acids 627-647 or 613-633 of the alternatively spliced forms of LIMK1), conjugated to KLH
This sequence is identical in rat LIMK1 and highly conserved (single amino acid substitution) in human LIMK1. It is not found in the LIMK2 isoform.
This sequence is identical in rat LIMK1 and highly conserved (single amino acid substitution) in human LIMK1. It is not found in the LIMK2 isoform.
アプリケーション
Anti-LIM Kinase 1 antibody is suitable for use in microarrays, protein densitometric assays (1:1000 using BPAE cell extracts ) and western blot (1:100, 000 using MDA-MB-231 cell lysates ).
生物化学的／生理学的作用
LIMK1 phosphorylates cofilin specifically at Serine 3. This inactivates its F-actin depolymerizing activity and leads to actin reorganization and accumulation of actin filaments. LIMK1 mRNA is highly expressed in the developing nervous system, heart and gut, and in adult brain and spinal cord, suggesting a role for LIMK-1 during neuronal cell differentiation. LIMK1 is localized in the presynaptic nerve ending during the synapse maturation event. LIMK1 also exhibits mitosis-specific activation.
物理的形状
0.01M PBS溶液 (pH 7.4, 15mMアジ化ナトリウム含有)。
免責事項
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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保管分類コード
12 - Non Combustible Liquids
WGK
WGK 2
引火点(°F)
Not applicable
引火点(℃)
Not applicable
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
Jan Code
L2290-200UL:
L2290-VAR:
L2290-BULK:
試験成績書（COA）
製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
Ora Bernard
The international journal of biochemistry & cell biology, 39(6), 1071-1076 (2006-12-26)
The members of the LIM kinase (LIMK) family, which include LIMK 1 and 2, are serine protein kinases involved in the regulation of actin polymerisation and microtubule disassembly. Their activity is regulated by phosphorylation of a threonine residue within the
Tissue specific characterisation of Lim-kinase 1 expression during mouse embryogenesis
Lindstrom NO, et al.
Gene Expression Patterns, 11(3-4), 221-232 (2011)
Mitosis-specific activation of LIM motif-containing protein kinase and roles of cofilin phosphorylation and dephosphorylation in mitosis
Amano T, et al.
The Journal of biological chemistry, 277(24), 22093-22102 (2002)
Brice V McConnell et al.
Molecular cancer, 10, 75-75 (2011-06-21)
LIM kinase 1 (LIMK1) is expressed in both cytoplasmic and nuclear compartments, and is a key regulator of cytoskeletal organization involved in cell migration and proliferation. LIMK1 levels are increased in several human cancers, with LIMK1 over-expression in prostate and
J Y Wang et al.
The Journal of comparative neurology, 416(3), 319-334 (1999-12-22)
LIM kinase 1 (LIMK1) is a cytoplasmic protein kinase that is highly expressed in neurons. In transfected cells, LIMK1 binds to the cytoplasmic tail of neuregulins and regulates the breakdown of actin filaments. To identify potential functions of LIMK1 in
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